A novel graphene oxide/chitosan foam incorporated with metal–organic framework stationary phase for simultaneous enrichment of glycopeptide and phosphopeptide with high efficiency

Autor:	Rong Liu, Wenqing Gao, Jiaqian Yang, Shun Zhang, Chenlu Wang, Jing Lin, Sijia Zhang, Jiancheng Yu, Keqi Tang
Rok vydání:	2022
Předmět:	Phosphopeptides Chitosan Glycopeptides Humans Graphite Hydrophobic and Hydrophilic Interactions Biochemistry Metal-Organic Frameworks Analytical Chemistry
Zdroj:	Analytical and Bioanalytical Chemistry. 414:2251-2263
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-021-03861-z
Popis:	A novel hydrophilic porous biocomposite was fabricated by incorporating graphene oxide (GO) @chitosan (CS) foam substrate (GO@CS@ZIF-8 foam) with ZIF-8 crystals in situ via a facile stirring method for simultaneous enrichment of glycopeptides and phosphopeptides from complex biological samples. The experimental results demonstrated that GO@CS@ZIF-8 foam exhibited favorable specificity for simultaneous enrichment of N-glycopeptides and phosphopeptides under the same condition for HRP and β-casein tryptic digest mixtures. The novel material was further applied to enriching both glycopeptides and phosphopeptides simultaneously from 4 μL complex human serum, and 423 N-glycopeptides and 40 phosphopeptides corresponding to 133 glycoproteins and 29 phosphoproteins were identified, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a98405d6be03d7d91f0b834e63376dbe https://doi.org/10.1007/s00216-021-03861-z Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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