Content of the Alternaria mycotoxin tenuazonic acid in food commodities determined by a stable isotope dilution assay
| Autor: | Stefan Asam, Martina Lichtenegger, Yang Liu, Michael Rychlik |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Detection limit
biology Relative standard deviation Reproducibility of Results Tenuazonic Acid Mycotoxins Toxicology Alternaria biology.organism_classification Whole wheat Microbiology Stable isotope dilution Sensitivity and Specificity Chemistry Techniques Analytical chemistry.chemical_compound chemistry Isotope Labeling Tenuazonic acid Fruit juice Food science Mycotoxin Food Analysis Biotechnology |
| Zdroj: | Mycotoxin research. 28(1) |
| ISSN: | 1867-1632 |
| Popis: | The Alternaria mycotoxin tenuazonic acid (TA) was quantified in fruit juices (n = 50), cereals (n = 12) and spices (n = 38) using a recently developed stable isotope dilution assay (SIDA). [(13) C6,(15) N]-TA was used as the internal standard. Method validation revealed low limits of detection (LODs) of 0.15 μg/kg (fruit juices), 1.0 μg/kg (cereals) and 17 μg/kg (spices). The respective limits of quantitation were about three times higher. Recovery was about 100% for all matrices. The precision (relative standard deviation of replicate analyses of naturally contaminated samples) was 4.2% (grape juice; 1.7 μg/kg), 3.5% (whole wheat flour; 36 μg/kg) and 0.9% (curry powder; 215 μg/kg). The median content of TA in the analyzed samples was 1.8 μg/kg (fruit juices), 16 μg/kg (cereals) and 500 μg/kg (spices). Positive samples amounted to 86% (fruit juices), 92% (cereals) and 87% (spices). |
| Databáze: | OpenAIRE |
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