Antibody biosensors for the measurement and characterization of soluble CD147 molecules
| Autor: | Saichit Khummuang, Nuchjira Takheaw, Kantinan Cheunsirikulchai, Supansa Pata, Watchara Kasinrerk, Witida Laopajon, Tanyaluck Kampoun |
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| Rok vydání: | 2017 |
| Předmět: |
Gene isoform
medicine.drug_class Immunology Cellular functions macromolecular substances Biosensing Techniques Monoclonal antibody 01 natural sciences medicine Immunology and Allergy Molecule Animals Humans biology Chemistry 010401 analytical chemistry technology industry and agriculture Antibodies Monoclonal General Medicine 0104 chemical sciences Biochemistry Monoclonal biology.protein Basigin Antibody Biosensor |
| Zdroj: | Asian Pacific journal of allergy and immunology. 36(3) |
| ISSN: | 0125-877X |
| Popis: | Background Soluble CD147 (sCD147) is the shed form of membrane-bound CD147, which is involved in the regulation of cellular functions. The presence of sCD147 in body fluids is associated with several diseases. Objective In this study, we aimed to establish antibody (Ab) biosensors for the simultaneous differential detection of the general and truncated forms of sCD147. Method By combining biolayer interferometry technology (BLItz) and different anti-CD147 monoclonal antibodies (mAbs) specific to different extracellular domains of the CD147 molecule, Ab-based biosensors were established to rapidly measure and characterize sCD147 isoforms. Results Two types of Ab-biosensors, desginated the single Ab-biosensor and double Ab-biosensor, were established for the measurment and characterization of sCD147 isoforms. For the single Ab-biosensor system, monoclonal antibodies specific for CD147 domain 1 (D1) or domain 2 (D2) were immobilized on the sensor tips and used for the quantification of sCD147 using a BLItz optical interferometric biosensor. For the double Ab-biosensor system, following the single Ab-biosensor step, secondary anti-CD147 mAbs specific for each domain of the CD147 molecule were added and monitored by a BLItz biosensor. By combining the results obtained from the single Ab- and double Ab-biosensors, sCD147 isoforms including the general form (D1 linked to D2) and the truncated forms (sCD147 containing D1 or D2) could be determined. Conclusions This method may be a beneficial tool for the determination of sCD147 isoforms for disease diagnosis and prognosis as well as for the definition of the cellular mechanisms of the immune system. |
| Databáze: | OpenAIRE |
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