Petatewalide B, a novel compound from Petasites japonicus with anti-allergic activity
| Autor: | Saeromi Kang, Dong-Soon Im, Jung-Min Kim, Jee H. Jung, Young-Geun Lee, Jung-Min Lee, Kyoung-Pil Lee, Soo-Jin Park, Hyung Ryong Moon, Young-Whan Choi |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Petasites Anti-Inflammatory Agents Nitric Oxide Synthase Type II Nitric Oxide Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor Anti-Allergic Agents Drug Discovery medicine Animals Mast Cells Pharmacology Mice Inbred BALB C biology Plant Extracts business.industry Degranulation Petasites japonicus Mast cell biology.organism_classification Molecular biology Asthma beta-N-Acetylhexosaminidases In vitro Rats Plant Leaves Nitric oxide synthase 030104 developmental biology medicine.anatomical_structure chemistry Cyclooxygenase 2 030220 oncology & carcinogenesis Immunology biology.protein Calcium business Sesquiterpenes |
| Zdroj: | Journal of Ethnopharmacology. 178:17-24 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance The giant butterbur Petasites japonicus is used to treat asthma and allergic diseases in traditional Korean, Japanese, and Chinese medicine. Aim of the study To elucidate the anti-allergic effect of Petasites genus, we studied effects of several compounds from Petasites japonicus leaves and found a novel bakkenolide-type sesquiterpine. In the present study, anti-allergic and anti-inflammatory effects of the new compound was examined using in vivo and in vitro experiments. Materials and methods The novel compound was isolated from Petasites japonicus leaves and named petatewalide B. Antigen-induced degranulation and Ca2+ mobilization were measured in RBL-2H3 mast cells by measuring β-hexosaminidase activity and fluorescence change of Ca2+ probe, fura-2. Induction of inducible nitric oxide synthase and cyclooxygenase 2 was measured by Western blotting in peritoneal macrophages. In addition, ovalbumin-induced asthma model was used for in vivo efficacy test of petatewalide B. Membrane potential was estimated by measuring fluorescence change of DiBAC in C6 glioma cells. Results Petatewalide B inhibited the antigen-induced degranulation of β-hexosaminidase in RBL-2H3 mast cells, but did not affect antigen-induced Ca2+ increase in the cells. Petatewalide B also showed inhibition of the LPS-induced induction of iNOS, but not COX-2 in mouse peritoneal macrophages. Nitric oxide production was also inhibited by petatewalide B in macrophages. In the ovalbumin-induced asthma model, petatewalide B strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. Petatewalide B increased the membrane potential of C6 glioma cells in a concentration-dependent manner. Conclusion Petatewalide B from Petasites genus not only has anti-allergic and anti-inflammatory effects but also induces a transient increase of membrane potential in C6 glioma cells. |
| Databáze: | OpenAIRE |
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