A histochemical study of the posterior silk glands ofBombyx moriduring metamorphosis from larvae to pupae using frozen sections
| Autor: | Kuniaki Hosono, Hajime Shiba, K Kawamoto, Tadafumi Kawamoto |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Histology
media_common.quotation_subject Apoptosis In situ hybridization Biology Polymerase Chain Reaction Gene Expression Regulation Enzymologic chemistry.chemical_compound Bombyx mori In Situ Nick-End Labeling Animals DAPI Metamorphosis In Situ Hybridization media_common Larva Frozen section procedure fungi Metamorphosis Biological Pupa General Medicine Bombyx biology.organism_classification Molecular biology Medical Laboratory Technology chemistry Caspases |
| Zdroj: | Biotechnic & Histochemistry. 89:145-152 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.3109/10520295.2013.830777 |
| Popis: | The fine structures of the whole bodies and the posterior silk glands of Bombyx mori during metamorphosis from larvae to pupae in the cocoon were preserved virtually without damage when frozen sections were prepared using an adhesive plastic film. We used frozen sections for histochemical and enzyme histochemistry to characterize the metamorphosis of the posterior silk glands. Frozen sections were stained with DAPI to observe nuclear changes, examined using the TUNEL method to detect DNA fragments, and investigated using in situ hybridization to detect B. mori caspase expression. Both DNA fragments and expression of B. mori caspase increased with progressing metamorphosis. The degeneration of the posterior silk gland during metamorphosis appears to be an apoptotic event. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|