Bone regeneration using the freshly isolated autologous stromal vascular fraction of adipose tissue in combination with calcium phosphate ceramics
Autor: | Christiaan M. ten Bruggenkate, Marco N. Helder, Engelbert A. J. M. Schulten, Henk-Jan Prins, Jenneke Klein-Nulend |
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Přispěvatelé: | Orale Celbiologie (ORM, ACTA), MKA VUmc (ORM, ACTA), Hematology laboratory, Oral and Maxillofacial Surgery / Oral Pathology, MOVE Research Institute, Orthopedic Surgery and Sports Medicine, Oral Cell Biology, Maxillofacial Surgery (VUmc) |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Calcium Phosphates Male medicine.medical_specialty Sinus Floor Augmentation Pathology Stromal cell Bone Regeneration medicine.medical_treatment Adipose tissue Mesenchymal Stem Cell Transplantation Regenerative Medicine 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Tissue Engineering and Regenerative Medicine medicine Maxilla Humans Bone regeneration Dental implant Aged Dental Implants business.industry Osteoid Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine X-Ray Microtomography Stromal vascular fraction Middle Aged Flow Cytometry Surgery 030104 developmental biology Adipose Tissue 030220 oncology & carcinogenesis Microscopy Electron Scanning Female business Developmental Biology |
Zdroj: | Prins, H J, Schulten, E A J M, ten Bruggenkate, C M, Klein-Nulend, J & Helder, M N 2016, ' Bone regeneration using the freshly isolated autologous stromal vascular fraction of adipose tissue in combination with calcium phosphate ceramics ', Stem Cells Translational Medicine, vol. 5, no. 10, pp. 1362-1374 . https://doi.org/10.5966/sctm.2015-0369 Stem Cells Translational Medicine, 5(10), 1362-1374. AlphaMed Press Prins, H-J, Schulten, E A J M, Ten Bruggenkate, C M, Klein-Nulend, J & Helder, M N 2016, ' Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics ', Stem Cells Translational Medicine, vol. 5, no. 10, pp. 1362-1374 . https://doi.org/10.5966/sctm.2015-0369 |
ISSN: | 2157-6580 2157-6564 |
Popis: | In patients undergoing maxillary sinus floor elevation (MSFE) for dental implant placement, bone substitutes are currently evaluated as alternatives for autologous bone. However, bone substitutes have only osteoconductive properties and lack osteoinductive potential. Therefore, this phase I study evaluated the potential additive effect on bone regeneration by the addition of freshly isolated, autologous but heterologous stromal vascular fraction (SVF), which is highly enriched with adipose stromal/stem cells when compared with native adipose tissue. From 10 patients, SVF was procured using automatic processing, seeded on either β-tricalcium phosphate (n = 5) or biphasic calcium phosphate carriers (n = 5), and used for MSFE in a one-step surgical procedure. Primary objectives were feasibility and safety. The secondary objective was efficacy, evaluated by using biopsies of the augmented area taken 6 months postoperatively, concomitant with dental implant placement. Biopsies were assessed for bone, graft, and osteoid volumes. No adverse effects were reported during the procedure or follow-up (≥3 years). Bone and osteoid percentages were higher in study biopsies (SVF supplemented) than in control biopsies (ceramic only on contralateral side), in particular in β-tricalcium phosphate-treated patients. Paired analysis on the six bilaterally treated patients revealed markedly higher bone and osteoid volumes using microcomputed tomography or histomorphometric evaluations, demonstrating an additive effect of SVF supplementation, independent of the bone substitute. This study demonstrated for the first time the feasibility, safety, and potential efficacy of SVF seeded on bone substitutes for MSFE, providing the first step toward a novel treatment concept that might offer broad potential for SVF-based regenerative medicine applications.SignificanceThis is the first-in-human study using freshly isolated, autologous adipose stem cell preparations (the stromal vascular fraction [SVF] of adipose tissue) applied in a one-step surgical procedure with calcium phosphate ceramics (CaP) to increase maxillary bone height for dental implantations. All 10 patients received CaP plus SVF on one side, whereas bilaterally treated patients (6 of 10) received CaP only on the opposite side. This allowed intrapatient evaluation of the potential added value of SVF supplementation, assessed in biopsies obtained after 6 months. Feasibility, safety, and potential efficacy of SVF for bone regeneration were demonstrated, showing high potential for this novel concept. |
Databáze: | OpenAIRE |
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