Multiplexed detection of foodborne pathogens based on magnetic particles
| Autor: | Susana Liébana, María Isabel Pividori, Delfina Brandão |
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| Rok vydání: | 2015 |
| Předmět: |
Bioengineering
Biosensing Techniques Real-Time Polymerase Chain Reaction medicine.disease_cause Immunomagnetic separation Disease Outbreaks Microbiology Foodborne Diseases Listeria monocytogenes medicine Food microbiology Multiplex Food components Molecular Biology Food poisoning Bacteria biology Immunomagnetic Separation General Medicine Microfluidic Analytical Techniques biology.organism_classification medicine.disease Salmonella enterica Food Microbiology Biotechnology |
| Zdroj: | New Biotechnology. 32:511-520 |
| ISSN: | 1871-6784 |
| Popis: | This paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes. End-point and real-time PCR, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of magnetic particles in these approaches. Although the immunomagnetic separation for capturing single bacteria from contaminating microflora and interfering food components has demonstrated to improve the performance on these approaches, the integration of magnetic particles for multiplex detection of bacteria is still in a preliminary stage and requires further studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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