Cutaneous and inflammatory response to long-term percutaneous implants of sphere-templated porous/solid poly(HEMA) and silicone in mice
| Autor: | Ge Zhao, Buddy D. Ratner, John E. Olerud, Marcia L. Usui, Andrew J. Marshall, Philip Fleckman, Max Maginness, Robert A. Underwood, Christine L. Glaister |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Dorsum
Time Factors Percutaneous Materials science Inflammatory response Silicones Biomedical Engineering Administration Cutaneous Methacrylate Article Biomaterials Mice chemistry.chemical_compound Silicone Implants Experimental Animals Porosity Polyhydroxyethyl Methacrylate Skin Inflammation Metals and Alloys Biomaterial Dermis Microspheres Mice Inbred C57BL Platelet Endothelial Cell Adhesion Molecule-1 chemistry Ceramics and Composites Female Epidermis Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. :1256-1268 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.34012 |
| Popis: | This study investigates mouse cutaneous responses to long-term percutaneously implanted rods surrounded by sphere-templated porous biomaterials engineered to mimic medical devices surrounded by a porous cuff. We hypothesized that keratinocytes would migrate through the pores and stop, permigrate, or marsupialize along the porous/solid interface. Porous/solid-core poly(2-hydroxyethyl methacrylate) [poly(HEMA)] and silicone rods were implanted in mice for 14 days, 1 month, 3 months, and 6 months. Implants with surrounding tissue were analyzed (immuno)histochemically by light microscopy. Poly(HEMA)/skin implants yielded better morphologic data than silicone implants. Keratinocytes at the poly(HEMA) interface migrated in two different directions. “Ventral” keratinocytes contiguous with the dermal-epidermal junction migrated into the outermost pores, forming an integrated collar surrounding the rods. ”Dorsal” keratinocytes appearing to emanate from the differentiated epithelial layer, extended upward along and into the exterior portion of the rod, forming an integrated sheath. Leukocytes persisted in poly(HEMA) and silicone pores for the duration of the study. Vascular and collagen networks within the poly(HEMA) pores matured as a function of time up to 3 months implantation. Nerves were not observed within the pores. Poly(HEMA) underwent morphological changes by 6 months of implantation. Marsupialization, foreign body encapsulation and infection were not observed in any implants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text