The effect of a temperature-sensitive poloxamer-alginate-CaCl2 mixture after strabismus surgery in a rabbit model
| Autor: | Woo Chan Park, Hye Mi Jung, Yoon Hyung Kwon, Woo Jin Jeung, Hee Bae Ahn, Sae Heun Rho, Mee Sook Roh, Won Yeol Ryu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Pathology Alginates medicine.medical_treatment H&E stain Tissue Adhesions Ophthalmologic Surgical Procedures Poloxamer Conjunctival Diseases Masson's trichrome stain Calcium Chloride Postoperative Complications Fibrosis Ophthalmology medicine Animals Saline business.industry Temperature medicine.disease eye diseases Staining Sclera Scleral Diseases Strabismus Disease Models Animal medicine.anatomical_structure Pediatrics Perinatology and Child Health Rabbits business Strabismus surgery Superior rectus muscle |
| Zdroj: | Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus. 17(5) |
| ISSN: | 1528-3933 |
| Popis: | Purpose To determine the efficacy of a temperature-sensitive poloxamer-alginate mixture in reducing adhesions after strabismus surgery in a rabbit model. Methods The superior rectus muscle was recessed in each of 36 eyes from 18 rabbits. One randomly assigned eye in each rabbit was treated with a poloxamer-alginate mixture (PA group); the other eye was treated with a subconjunctival injection of saline (control group). The adhesions between the superior rectus muscle, sclera, and conjunctiva were clinically evaluated by a masked observer at 1 day, 1 week, and 4 weeks after surgery. Inflammation was analyzed by hematoxylin and eosin staining and anti-CD11b staining. Late fibrosis was assessed by the Masson trichrome and α-smooth muscle actin staining. Adhesion, inflammation, and fibrosis were graded on a scale of 0-4. Results There was no significant between-group difference in the degree of adhesion at 1 day and 4 weeks after surgery. However, the degree of adhesion in the PA group was lower than that in the control group at postoperative week 1 ( P P > 0.05). At postoperative week 4, inflammatory cell infiltration was reduced in the PA group ( P = 0.046). A significant between-group difference in late fibrosis at postoperative week 4 was observed through the Masson trichrome ( P = 0.024) and α-smooth muscle actin staining ( P = 0.025). Conclusions The poloxamer-alginate mixture significantly decreased adhesion after strabismus surgery in a rabbit model. Additional studies are warranted to evaluate the use of this mixture in preventing postoperative adhesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect