Aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet
| Autor: | Maria José Figueras, Arturo Monera, Antonio J. Martínez-Murcia, Evelyne Brambilla, Anabel Alperi, Erko Stackebrandt, Roxana Beaz-Hidalgo |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Phylogenetic tree Sequence analysis Molecular Sequence Data Fresh Water General Medicine Sequence Analysis DNA Ribosomal RNA Biology biology.organism_classification 16S ribosomal RNA Microbiology Housekeeping gene Bacterial Typing Techniques Phenotype Aeromonas Phylogenetics Genes Bacterial RNA Ribosomal 16S Multilocus sequence typing Ecology Evolution Behavior and Systematics Phylogeny Multilocus Sequence Typing |
| Zdroj: | International journal of systematic and evolutionary microbiology. 61(Pt 2) |
| ISSN: | 1466-5034 |
| Popis: | Two freshwater isolates (WB4.1-19T and WB4.4-101), sharing 99.9 % 16S rRNA gene sequence similarity, were highly related to Aeromonas sobria (99.7 % similarity; 6 bp differences). A phylogenetic tree derived from a multi-locus phylogenetic analysis (MLPA) of the concatenated sequences of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of Aeromonas molluscorum and Aeromonas bivalvium. The DNA–DNA reassociation value between the two new isolates was 89.3 %. Strain WB4.1-19T had a DNA–DNA relatedness value of Aeromonas. It is concluded that the two new strains represent a novel species of the genus Aeromonas, for which the name Aeromonas rivuli sp. nov. is proposed, with the type strain WB4.1-19T (=CECT 7518T=DSM 22539T=MDC 2511T). |
| Databáze: | OpenAIRE |
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