Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
| Autor: | Erja Kerkelä, Kim Bergström, Jukka Jolkkonen, Matti Korhonen, Johanna Nystedt, Mari Raki, Joonas Khabbal, Bhimashankar Mitkari, Saara Laitinen, Ville Mikkonen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biodistribution Stromal cell Xenotransplantation medicine.medical_treatment External carotid artery Biomedical Engineering lcsh:Medicine Bone Marrow Cells Mesenchymal Stem Cell Transplantation medicine.artery medicine Animals Rats Wistar Stroke Transplantation medicine.diagnostic_test business.industry Mesenchymal stem cell lcsh:R Mesenchymal Stem Cells Cell Biology medicine.disease Rats Radiography Heterografts business Emission computed tomography |
| Zdroj: | Cell Transplantation, Vol 24 (2015) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | Intra-arterial (IA) delivery of bone marrow-derived mesenchymal stem cells (BM-MSCs) has shown potential as a minimally invasive therapeutic approach for stroke. The aim of the present study was to determine the whole-body biodistribution and clearance of technetium-99m (99mTc)-labeled rat and human BM-MSCs after IA delivery in a rat model of transient middle cerebral artery occlusion (MCAO) using single-photon emission computed tomography (SPECT). Our hypothesis was that xenotransplantation has a major impact on the behavior of cells. Male RccHan: Wistar rats were subjected to sham operation or MCAO. Twenty-four hours after surgery, BM-MSCs (2×106 cells/animal) labeled with 99mTc were infused into the external carotid artery. Whole-body SPECT images were acquired 20 min, 3 h, and 6 h postinjection, after which rats were sacrificed, and organs were collected and weighed for measurement of radioactivity. The results showed that the majority of the cells were located in the brain and especially in the ipsilateral hemisphere immediately after cell infusion both in sham-operated and MCAO rats. This was followed by fast disappearance, particularly in the case of human cells. At the same time, the radioactivity signal increased in the spleen, kidney, and liver, the organs responsible for destroying cells. Further studies are needed to demonstrate whether differential cell behavior has any functional impact. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|