Antimicrobial Susceptibility and Multilocus Sequence Typing ofListeria monocytogenesIsolated Over 11 Years from Food, Humans, and the Environment in Italy
Autor: | Angela Miccolupo, Laura Latorre, Marta Caruso, Frederique Pasquali, Antonio Parisi, Giovanni Normanno, G. Santagada, Laura Difato, Rosa Fraccalvieri |
---|---|
Přispěvatelé: | Marta Caruso, Rosa Fraccalvieri, Frederique Pasquali, Gianfranco Santagada, Laura M. Latorre, Laura M. Difato, Angela Miccolupo, Giovanni Normanno, Antonio Parisi |
Rok vydání: | 2020 |
Předmět: |
Serotype
040301 veterinary sciences medicine.drug_class Antibiotics multilocus sequence typing Food Contamination Biology Serogroup medicine.disease_cause Applied Microbiology and Biotechnology Microbiology antimicrobial susceptibility 0403 veterinary science 03 medical and health sciences Minimum inhibitory concentration Antibiotic resistance Listeria monocytogenes Anti-Bacterial Agent Drug Resistance Bacterial Prevalence medicine Listeria monocytogene 0303 health sciences Microbial Sensitivity Test 030306 microbiology Clindamycin Environmental Exposure 04 agricultural and veterinary sciences Foodborne Disease serotyping DNA Environmental Ciprofloxacin Italy Food Microbiology Multilocus sequence typing Animal Science and Zoology Human Food Science medicine.drug |
Zdroj: | Foodborne Pathogens and Disease. 17:284-294 |
ISSN: | 1556-7125 1535-3141 |
Popis: | Due to the increasing number of studies reporting the detection of antimicrobial-resistant isolates of Listeria monocytogenes, we sought to determine the antimicrobial susceptibility of L. monocytogenes isolates collected in Italy and find potential correlations to their serotypes and multilocus sequence types (MLST). The antimicrobial susceptibility of 317 L. monocytogenes isolates collected from food, humans, and the environment from 1998 to 2009 was assessed by minimum inhibitory concentration (MIC). Serotyping and MLST was also performed on all isolates. Potential correlations among antimicrobial resistance profiles, serotyping, and MLST were statistically evaluated. Twenty-four percent of L. monocytogenes isolates were resistant to oxacillin, 28.7% intermediate to clindamycin, and 24.3% to ciprofloxacin. The majority of isolates with elevated MIC to oxacillin was of environmental origin and belonged to serotype 4b/4e and ST2. Isolates with intermediate MIC values to clindamycin and ciprofloxacin were mostly of food and human origin and belonged to serotype 4b/4e and ST9. Regarding the time frame of isolate collection, comparing the last 3 years (2007-2009) to previous years (1998-2006), an increase was observed in the percentage of resistant and intermediate isolates per year. This trend strongly suggests the need for increasing attention on the prevalence of antimicrobial resistance in L. monocytogenes in Italy. To predict future resistance trends, the monitoring of clinical intermediate resistance might represent a useful tool especially for antibiotics associated to multiple-step mechanisms of acquired resistance. A specific focus should be addressed to antimicrobial-resistant isolates of serotype 4b, repeatedly associated with food-borne outbreaks. |
Databáze: | OpenAIRE |
Externí odkaz: |