Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma

Autor: Markéta Hermanová, Sylva Hotárková, Peter Múdry, Jaroslav Sterba, Iva Staniczková Zambo, Marcela Vesela, Tetiana Shatokhina, Jiri Jarkovsky, Marek Handl
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
Male
Pathology
medicine.medical_specialty
Adolescent
Clinicopathological correlation
Antigens
Differentiation
Myelomonocytic
lcsh:Medicine
Pilot Projects
Receptors
Cell Surface
Sarcoma
Ewing
Biology
cd163
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Young Adult
0302 clinical medicine
stomatognathic system
Antigens
CD
Cell Line
Tumor
medicine
Tumor Microenvironment
Humans
Child
skin and connective tissue diseases
Cell Proliferation
CD68
tumor associated macrophages
Macrophages
lcsh:R
medicine.disease
cd68
030104 developmental biology
030220 oncology & carcinogenesis
Localized disease
Child
Preschool
immunohistochemistry
Immunohistochemistry
Female
Sarcoma
prognosis
Carcinogenesis
CD163
hormones
hormone substitutes
and hormone antagonists
ewing sarcoma
Zdroj: Biomedical Papers, Vol 162, Iss 1, Pp 54-60 (2018)
ISSN: 1804-7521
1213-8118
Popis: Aims: Tumor-associated macrophages (TAMs) are known markers playing complex roles in tumorigenesis. However, the function of TAMs in a variety of malignancies is not yet fully understood. The aim of this pilot study was to quantify the density of TAMs in Ewing sarcoma and to determine the correlation between TAMs and clinicopathological parameters. Methods: Using immunohistochemistry, the expressions of CD68 and CD163 were examined in 24 tissue samples of Ewing sarcoma of bone. The density of CD68 and CD163-positive TAMs was analyzed quantitatively and semi-quantitatively and statistically correlated with clinical parameters. Results: CD163-positive TAMs outnumbered CD68-positive cells (median of 130 vs 96, respectively). No statistically significant relatio nship was found between density of CD68-positive cells, clinical parameters or prognosis. However, high levels of CD163-positive TAMs were associated with localized disease ( P =0.008). In cases with a higher density of CD163-positive cells, a trend toward longer survival was revealed ( P =0.063). Conclusion: This is the first study that has quantified CD163 expression in TAMs in Ewing sarcoma and showed its possible prognostic value. CD163 was confirmed to be a more specific marker of macrophages than CD68. CD163 is not an exclusive hallmark of M2 macrophages.
Databáze: OpenAIRE
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