Function of the mitochondrial outer membrane as a diffusion barrier in health and diseases
| Autor: | Evelin Seppet, Sonata Trumbeckaite, Jens R. Opalka, F. N. Gellerich, C. Neuhoff, Stephan Zierz, H. N. Rasmussen |
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| Rok vydání: | 2000 |
| Předmět: |
Mitochondrial intermembrane space
Translocase of the outer membrane Apoptosis Cytochrome c Group Mitochondria Liver Mitochondrion Biology Biochemistry Mitochondria Heart Diffusion Cytosol Adenine nucleotide Animals Inner mitochondrial membrane Intracellular Membranes Saponins Cell biology Cell Compartmentation Mitochondria Rats Adenosine Diphosphate Oxygen Reperfusion Injury Rabbits Bacterial outer membrane Intermembrane space |
| Zdroj: | Biochemical Society transactions. 28(2) |
| ISSN: | 0300-5127 |
| Popis: | The mitochondrial outer membrane separates the intermembrane space from the cytosol. The whole exchange of metabolites, cations and information between mitochondria and the cell occurs through the outer membrane. Experimental evidence is reviewed supporting the hypothesis of dynamic ADP compartmentation within the intermembrane space. The outer membrane creates a diffusion barrier for small molecules (adenine nucleotides, creatine phosphate, creatine etc.) causing rate-dependent concentration gradients as a prerequisite for the action of ADP shuttles via creatine kinases or adenylate kinases. If the outer membrane becomes leaky, cytochrome c and apoptosis-inducing factor can be released, leading to apoptosis, and as a bioenergetic consequence the cytosolic phosphorylation potential decreases. Leaky outer membranes can be detected in saponin-skinned fibres with spectrophotometric and oxygraphic methods. This is of special interest in respect to acute impairment of mitochondria during ischaemia/reperfusion. |
| Databáze: | OpenAIRE |
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