Role of BASIC PENTACYSTEINE transcription factors in a subset of cytokinin signaling responses
| Autor: | Markus Schmid, Klaus Harter, Joseph J. Kieber, Luise H. Brand, Andreas Hecker, Dierk Wanke, Carly Shanks, G. Eric Schaller, Silvio Collani, Chia Yi Cheng |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Plant growth Cytokinins Arabidopsis Plant Science Genes Plant 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Transcription (biology) Genetics Transcription factor biology Arabidopsis Proteins fungi food and beverages Cell Biology biology.organism_classification Cell biology DNA-Binding Proteins 030104 developmental biology Cytokinin signaling chemistry Glucosyltransferases Cytokinin human activities Signal Transduction Transcription Factors |
| Zdroj: | The Plant Journal. 95:458-473 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.13962 |
| Popis: | Cytokinin plays diverse roles in plant growth and development, generally acting by modulating gene transcription in target tissues. The type-B Arabidopsis response regulators (ARR) transcription factors have emerged as primary targets of cytokinin signaling and are required for essentially all cytokinin-mediated changes in gene expression. The diversity of cytokinin function is likely imparted by the activity of various transcription factors working with the type-B ARRs to alter specific sets of target genes. One potential set of co-regulators modulating the cytokinin response are the BARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC) family of plant-specific transcription factors. Here, we show that disruption of multiple BPCs results in reduced sensitivity to cytokinin. Further, the BPCs are necessary for the induction of a subset of genes in response to cytokinin. We identified direct in vivo targets of BPC6 using ChIP-Seq and found an enrichment of promoters of genes differentially expressed in response to cytokinin. Further, a significant number of BPC6 regulated genes are also direct targets of the type-B ARRs. Potential cis-binding elements for a number of other transcription factors linked to cytokinin action are enriched in the BPC binding fragments, including those for the cytokinin response factors (CRFs). In addition, several BPCs interact with a subset of type-A ARRs. Consistent with these results, a significant number of genes whose expression is altered in bpc mutant roots are also mis-expressed in crf1,3,5,6 and type-A arr3,4,5,6,7,8,9,15 mutant roots. These results suggest that the BPCs are part of a complex network of transcription factors that are involved in the response to cytokinin. |
| Databáze: | OpenAIRE |
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