Screening of variables influencing the clavulanic acid production by Streptomyces DAUFPE 3060 strain
| Autor: | Ana Lúcia Figueiredo Porto, José L. Lima-Filho, Daniela Viana, Adalberto Pessoa-Junior, M. N. Carneiro-Cunha, Janete Magali de Araújo, B. Barros-Neto, Attilio Converti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Glycerol
Time Factors Nitrogen Flour Bioengineering Industrial fermentation Soybean flour Applied Microbiology and Biotechnology Biochemistry Streptomyces Clavulanic acid Screening of variables Fermentation law.invention chemistry.chemical_compound Erlenmeyer flask Bioreactors law medicine Food science Biomass Molecular Biology biology Chemistry SOJA food and beverages Substrate (chemistry) Fractional factorial design General Medicine Hydrogen-Ion Concentration biology.organism_classification Carbon Biotechnology medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28 degrees C, 30 degrees C, and 32 degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32 degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%. |
| Databáze: | OpenAIRE |
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