Biosynthesis of Rishirilide B
| Autor: | Julia Wunsch-Palasis, Philipp Schwarzer, Andreas Bechthold, Thomas Paululat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Stereochemistry Streptomyces rishiriensis Streptomyces bottropensis 01 natural sciences Biochemistry Microbiology Streptomyces Article 03 medical and health sciences chemistry.chemical_compound Polyketide streptomyces rishirilide biosynthesis polyketides Biosynthesis Gene cluster Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Streptomyces albus biology 010405 organic chemistry Chemistry lcsh:RM1-950 biology.organism_classification 0104 chemical sciences lcsh:Therapeutics. Pharmacology 030104 developmental biology Infectious Diseases Methyl group |
| Zdroj: | Antibiotics; Volume 7; Issue 1; Pages: 20 Antibiotics, Vol 7, Iss 1, p 20 (2018) Antibiotics |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics7010020 |
| Popis: | Rishirilide B was isolated from Streptomyces rishiriensis and Streptomyces bottropensis on the basis of its inhibitory activity towards alpha-2-macroglobulin. The biosynthesis of rishirilide B was investigated by feeding experiments with different 13C labelled precursors using the heterologous host Streptomyces albus J1074::cos4 containing a cosmid encoding of the gene cluster responsible for rishirilide B production. NMR spectroscopic analysis of labelled compounds demonstrate that the tricyclic backbone of rishirilide B is a polyketide synthesized from nine acetate units. One of the acetate units is decarboxylated to give a methyl group. The origin of the starter unit was determined to be isobutyrate. |
| Databáze: | OpenAIRE |
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