Comprehensive Molecular Characterization of a Cisplatin-Specific Monoclonal Antibody
| Autor: | Stefanie Ickert, Jürgen Thomale, Johanna Hofmann, Michael W. Linscheid, Sebastian Beck, Ulrike Hochkirch, Lena Ruhe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Glycan medicine.drug_class Electrospray ionization Medizin Oligosaccharides Pharmaceutical Science Antigen-Antibody Complex Monoclonal antibody Mass spectrometry DNA Adducts Epitopes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polysaccharides Tandem Mass Spectrometry Drug Discovery medicine Native state Molecular Structure biology Protein primary structure Antibodies Monoclonal Molecular biology 030104 developmental biology chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 030220 oncology & carcinogenesis biology.protein Molecular Medicine Cisplatin Antibody DNA |
| Popis: | Despite their immense and rapidly increasing importance as analytical tools or therapeutic drugs, the detailed structural features of particular monoclonal antibodies are widely unknown. Here, an antibody already in use for diagnostic purposes and for molecular dosimetry studies in cancer therapy with very high affinity and specificity for cisplatin-induced DNA modifications was studied extensively. The molecular structure and modifications as well as the antigen specificity were investigated mainly by mass spectrometry. Using nano electrospray ionization mass spectrometry, it was possible to characterize the antibody in its native state. Tandem-MS experiments not only revealed specific fragments but also gave information on the molecular structure. The detailed primary structure was further elucidated by proteolytic treatment with a selection of enzymes and high resolution tandem-MS. The data were validated by comparison with known antibody sequences. Then, the complex glycan structures bound to the antibody were characterized in all detail. The Fc-bound oligosaccharides were released enzymatically and studied by matrix-assisted laser desorption/ionization mass spectrometry. Overall 16 different major glycan structures were identified. The binding specificity of the antibody was investigated by applying synthetic single and double stranded DNA oligomers harboring distinct Pt adducts. The antibody-antigen complexes were analyzed by mass spectrometry under native conditions. The stability of the complex with double stranded DNA was also investigated. |
| Databáze: | OpenAIRE |
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