Construction and expression of recombined human AFP eukaryotic expression vector
| Autor: | Hongmei Zhang, Bo-Rong Pan, Li-Wang Zhang, Jun Ren, Stephen M Festein, Liang Zhang, Zhong-Hua Wang, Yang-Lin Pan, Yan-Jun Zhang, Xiaoming Si, Bin Jin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Liver Cancer
Carcinoma Hepatocellular DNA Complementary Genetic enhancement Genetic Vectors Fluorescent Antibody Technique Gene Expression CHO Cells Biology Immunofluorescence Transfection law.invention Antigen law Cricetinae Gene expression medicine Animals Humans Vector (molecular biology) neoplasms medicine.diagnostic_test Chinese hamster ovary cell digestive oral and skin physiology Liver Neoplasms Gastroenterology General Medicine Genetic Therapy Molecular biology digestive system diseases Recombinant Proteins embryonic structures Recombinant DNA alpha-Fetoproteins |
| Popis: | AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC). METHODS: The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods. RESULTS: The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/mL AFP antigen was detected in the supernatant of AFP-CHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P < 0.05). AFP antigen molecule was observed in the plasma of AFP-CHO by immunofluorescence staining. CONCLUSION: The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|