Analysis of the long-term actions of gabapentin and pregabalin in dorsal root ganglia and substantia gelatinosa
| Autor: | Naren Ganesan, Aquilino Lantero, James E. Biggs, Paul A. Boakye, Peter A. Smith, Klaus Ballanyi, Patrick L. Stemkowski |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Patch-Clamp Techniques Cyclohexanecarboxylic Acids Gabapentin Physiology Pregabalin Pharmacology gamma-Aminobutyric acid Rats Sprague-Dawley Tissue Culture Techniques Substantia gelatinosa Ganglia Spinal medicine Animals Amines Cells Cultured gamma-Aminobutyric Acid Neurons Analgesics Microscopy Confocal Voltage-dependent calcium channel business.industry General Neuroscience Excitatory Postsynaptic Potentials Nerve injury Calcium Channel Blockers Immunohistochemistry Inhibitory Postsynaptic Potentials nervous system Substantia Gelatinosa Neuropathic pain Excitatory postsynaptic potential Calcium Calcium Channels medicine.symptom business Neuroscience medicine.drug |
| Zdroj: | Journal of Neurophysiology. 112:2398-2412 |
| ISSN: | 1522-1598 0022-3077 |
| DOI: | 10.1152/jn.00168.2014 |
| Popis: | The α2δ-ligands pregabalin (PGB) and gabapentin (GBP) are used to treat neuropathic pain. We used whole cell recording to study their long-term effects on substantia gelatinosa and dorsal root ganglion (DRG) neurons. Spinal cord slices were prepared from embryonic day 13 rat embryos and maintained in organotypic culture for >5 wk (neuronal age equivalent to young adult rats). Exposure of similarly aged DRG neurons (dissociated and cultured from postnatal day 19 rats) to GBP or PGB for 5–6 days attenuated high-voltage-activated calcium channel currents (HVA ICa). Strong effects were seen in medium-sized and in small isolectin B4-negative (IB4−) DRG neurons, whereas large neurons and small neurons that bound isolectin B4 (IB4+) were hardly affected. GBP (100 μM) or PGB (10 μM) were less effective than 20 μM Mn2+ in suppression of HVA ICa in small DRG neurons. By contrast, 5–6 days of exposure to these α2δ-ligands was more effective than 20 μM Mn2+ in reducing spontaneous excitatory postsynaptic currents at synapses in substantia gelatinosa. Spinal actions of gabapentinoids cannot therefore be ascribed to decreased expression of HVA Ca2+ channels in primary afferent nerve terminals. In substantia gelatinosa, 5–6 days of exposure to PGB was more effective in inhibiting excitatory synaptic drive to putative excitatory neurons than to putative inhibitory neurons. Although spontaneous inhibitory postsynaptic currents were also attenuated, the overall long-term effect of α2δ-ligands was to decrease network excitability as monitored by confocal Ca2+ imaging. We suggest that selective actions of α2δ-ligands on populations of DRG neurons may predict their selective attenuation of excitatory transmission onto excitatory vs. inhibitory neurons in substantia gelatinosa. |
| Databáze: | OpenAIRE |
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