Transcription profile analysis of the endometrium revealed molecular markers of the personalized ‘window of implantation’ during in vitro fertilization
Autor: | V K Bozhenko, Vladimir Naumov, O.V. Burmenskaya, Dmitry Yu. Trofimov, V. Yu. Smolnikova, E A Kalinina, P I Borovikov, I. E. Korneeva, Е P Beyk, N. Aleksandrova, A E Donnikov |
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Rok vydání: | 2017 |
Předmět: |
Adult
0301 basic medicine Infertility Endocrinology Diabetes and Metabolism medicine.medical_treatment Gene Expression Fertilization in Vitro Biology Endometrium Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Pregnancy Transcription (biology) medicine Humans Embryo Implantation Gene 030219 obstetrics & reproductive medicine In vitro fertilisation Gene Expression Profiling Obstetrics and Gynecology Embryo medicine.disease 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction PAEP Female Infertility Female Biomarkers |
Zdroj: | Gynecological Endocrinology. 33:22-27 |
ISSN: | 1473-0766 0951-3590 |
Popis: | To determine the most informative markers for assessing the functional state of endometrium during the 'window of implantation' and creating a model for assessment of the readiness of endometrium for embryo implantation. Forty-seven women with tubal infertility and a successful IVF pregnancy participated in the study. Pipelle endometrial sample was performed during the supposed 'window of implantation' in natural cycle with subsequent histological study, and transcriptional profile of genes GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, POSTN determined by real-time quantitative polymerase chain reaction (qRT-PCR). Differences in the level of mRNA expression of all the studied genes in the receptive endometrium were found in comparison to the prereceptive one, which allowed us to classify two functional states of the endometrium. The results of histological examination responded to the stage of maturation of the endometrium in 78.7% of cases. Receptive endometrial status can be determined based on the integral evaluation of mRNA expression level of 4 PAEP, DPP4, MSX1, and HLA-DOB genes. The model for determining a personalized `window implantation' is offered for practical application in ART. |
Databáze: | OpenAIRE |
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