Interleukin polymorphisms and differential methylation status in gastric cancer: an association with Helicobacter pylori infection
| Autor: | Eduardo Henrique Cunha Neves-Filho, Débora Menezes da Costa, Silvia Helena Barem Rabenhorst, Markênia Kélia Santos Alves |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Biology medicine.disease_cause Epigenesis Genetic Helicobacter Infections Bacterial Proteins Stomach Neoplasms Genotype Genetics medicine Humans CagA Promoter Regions Genetic Genotyping Cyclin-Dependent Kinase Inhibitor p16 Genetic Association Studies Aged Polymorphism Genetic Helicobacter pylori Interleukin-6 Interleukin Cancer Methylation DNA Methylation Middle Aged medicine.disease Cyclooxygenase 2 DNA methylation Immunology Cancer research Female Carcinogenesis |
| Zdroj: | Epigenomics. 5:167-175 |
| ISSN: | 1750-192X 1750-1911 |
| DOI: | 10.2217/epi.13.7 |
| Popis: | Aim: Interleukin polymorphisms and Helicobacter pylori infection are believed to play critical roles in DNA methylation, a process frequently associated with carcinogenesis. The aim of this study was to determine the associations between interleukin polymorphisms and methylation status of three genes related to gastric cancer. Furthermore, the influence of the H. pylori strains was evaluated. Materials & methods: 75 gastric tumor samples had the DNA extracted for interleukin polymorphisms genotyping by PCR-RFLP, promoter methylation by MS-PCR and detection and subtyping of H. pylori by PCR. Results: In the cardia tumors, methylation in the COX-2 promoter was associated with IL1RN*2 (p = 0.015), and the associated genotypes IL1B511T + IL1RN*2 seem to be important in the methylation of COX-2 (p = 0.013), especially in the presence of cagA+ (p = 0.026) and vacAs1 (p = 0.025) H. pylori strains. The associated genotypes IL6 CC+TNF GG seem to be involved in the unmethylation of CDKN2A (p = 0.046), along with H. pylori cagA+ infection. Conclusion: DNA methylation in gastric cancer seems to be influenced by the presence of interleukin polymorphisms and by the H. pylori cagA/vacAs1m1 strains. |
| Databáze: | OpenAIRE |
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