Systematic gene targeting on the X chromosome of Drosophila melanogaster
| Autor: | Nicole Beinert, Meike Werner, Ulrich Schäfer, Herbert Jäckle, Gordon Dowe, Ho-Ryun Chung |
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| Rok vydání: | 2004 |
| Předmět: |
Genetics
X Chromosome biology ved/biology ved/biology.organism_classification_rank.species Chromosome Mapping Gene targeting Genes Insect biology.organism_classification Genome Mutagenesis Insertional Drosophila melanogaster Gene Targeting DNA Transposable Elements Melanogaster Animals Genes Lethal Chromosome Deletion Model organism Gene Genetics (clinical) X chromosome Genetic screen |
| Zdroj: | Chromosoma. 113:271-275 |
| ISSN: | 1432-0886 0009-5915 |
| Popis: | The genome of the model organism Drosophila melanogaster has been sequenced and annotated. Based on this groundwork, we performed a systematic genetic screen of the D. melanogaster X chromosome, which carries about one sixth of the genes of the organism. We generated a collection of single P-element insertions to provide genetic and molecular access to virtually all X-chromosomal genes. The study complements earlier work designed to systematically identify vital genes on the X chromosome by targeting transcription units which are phenotypically silent. We describe single UAS sequence-bearing P-element insertions throughout the X chromosome, which allows one to express the tagged genes under control of tissue/organ-directed GAL4 activity. In addition, the present collection of single insertion lines provides a tool to generate chromosomal deletions which are on average less than 33 kb in size. |
| Databáze: | OpenAIRE |
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