Myrtle berry seed aqueous extract inhibits human neutrophil myeloperoxidase in vitro and attenuates acetic acid-induced ulcerative colitis in rats

Autor: Haifa Tounsi, Abdelaziz Souli, Mohsen Sakly, Mohamed-Amine Jabri, Jamel El-Benna, Karim Hosni, Kais Rtibi, Lamjed Marzouki, Hichem Sebai
Přispěvatelé: Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Université de Jendouba (UJ), Laboratoire d'Epidémiologie Moléculaire et de Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04), Université Tunis El Manar (UTM)-Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire des Substances Naturelles (LR02INRAP10), Institut National de Recherche et d'Analyse Physico-chimique (Ariana, Tunisie) (INRAP), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Faculté des Sciences de Bizerte [Université de Carthage], Université de Carthage - University of Carthage-Université de Carthage - University of Carthage, Université de Tunis El Manar (UTM)-Institut Pasteur de Tunis, Institut National de Recherche et d'Analyse Physico-chimique [Ariana, Tunisie] (INRAP)
Rok vydání: 2015
Předmět:
Zdroj: RSC Advances
RSC Advances, Royal Society of Chemistry, 2015, 5 (80), pp.64865-64877. ⟨10.1039/c5ra07172k⟩
ISSN: 2046-2069
DOI: 10.1039/c5ra07172k
Popis: International audience; We aimed in the present study to investigate the protective effect of a myrtle (Myrtus communis L.) berry seed aqueous extract (MBSAE) on acetic acid (AA)-induced colitis in rats as well as the mechanism implicated in this coli-protection. The use of the LC/MS technique allowed us to identify 18 phenolic compounds in the MBSAE. Secondly, we found that the MBSAE inhibited the luminol-amplified chemiluminescence of resting neutrophils and N-formyl-methionylleucyl-phenylalanine (fMLF) or phorbolmyristate acetate (PMA) stimulated neutrophils in a dose-dependent manner. The MBSAE had no effect on superoxide anions, but it inhibited H2O2 production in the cell free system stimulated with horseradish peroxidase (HRPO) and MPO release from the neutrophils. In vivo, the pre-treatment of rats with sulfasalazine (100 mg kg(-1)) and the MBSAE (25, 50, and 100 mg kg(-1)) significantly reduced AA-induced colonic mucosa lesions as well as histopathological changes. The MBSAE counteracted AA-induced lipid peroxidation and the depletion of the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also found that the myrtle extract inhibited the increase of the plasma scavenging activity (PSA) and preserved the content of non-enzymatic antioxidants such as sulfhydryl groups (-SH) and reduced glutathione (GSH). More importantly, acetic acid administration increased colonic hydrogen peroxide (H2O2), free iron and calcium levels, while the MBSAE pre-treatment reversed all intracellular mediator perturbations. In conclusion, our data suggests that the MBSAE exerted a potential protective effect against AA-induced injury and oxidative stress in the rat colon. This coli-protection might be related in part to its antioxidant and ROS scavenging activities or by negatively regulating Fenton reaction components such as H2O2 and free iron, which are known to lead to cytotoxicity mediated by intracellular calcium deregulation.
Databáze: OpenAIRE
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