Concerns about the feasibility of using 'precision guided sterile males' to control insects
| Autor: | Marc J. B. Vreysen, Jérémy Bouyer |
|---|---|
| Přispěvatelé: | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Insect Pest Control Laboratory (IPC laboratory), Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-International Atomic Energy Agency [Vienna] (IAEA), Bouyer, Jérémy |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
CRISPR-Cas9 genome editing Infertilité mâle Insecta Molecular biology Genome Insect General Physics and Astronomy technique de laboratoire L73 - Maladies des animaux Méthode de lutte Sexual Behavior Animal 0302 clinical medicine Aedes aegypti lcsh:Science ComputingMilieux_MISCELLANEOUS Animal biology Gene Editing 0303 health sciences Multidisciplinary biology [SDV.BA]Life Sciences [q-bio]/Animal biology Biological techniques Lâcher d'insectes stériles Aedes albopictus Drosophila Female Population Control L72 - Organismes nuisibles des animaux Biotechnology RNA Guide Kinetoplastida Science 030231 tropical medicine Models Biological General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Matters Arising Animal transgénique Biologie animale Genetics Animals Humans Pest Control Biological 030304 developmental biology business.industry Diptera General Chemistry biology.organism_classification Insect Vectors Genetic engineering Feasibility Studies lcsh:Q CRISPR-Cas Systems U30 - Méthodes de recherche business |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-2 (2019) Nature Communications Nature Communications, Nature Publishing Group, 2019, 10, 2 p. ⟨10.1038/s41467-019-11616-9⟩ Nature Communications (10), 2 p.. (2019) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-11616-9 |
| Popis: | The sterile insect technique (SIT) is an environmentally safe and proven technology to suppress wild populations. To further advance its utility, a novel CRISPR-based technology termed precision guided SIT (pgSIT) is described. PgSIT mechanistically relies on a dominant genetic technology that enables simultaneous sexing and sterilization, facilitating the release of eggs into the environment ensuring only sterile adult males emerge. Importantly, for field applications, the release of eggs will eliminate burdens of manually sexing and sterilizing males, thereby reducing overall effort and increasing scalability. Here, to demonstrate efficacy, we systematically engineer multiple pgSIT systems in Drosophila which consistently give rise to 100% sterile males. Importantly, we demonstrate that pgSIT-generated sterile males are fit and competitive. Using mathematical models, we predict pgSIT will induce substantially greater population suppression than can be achieved by currently-available self-limiting suppression technologies. Taken together, pgSIT offers to potentially transform our ability to control insect agricultural pests and disease vectors. |
| Databáze: | OpenAIRE |
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