Indications for an immune-mediated etiology of idiopathic sensory neuronopathy
| Autor: | Leonard H. van den Berg, Nicolette C. Notermans, P. R. Bär, Gert van Dijk, John H. J. Wokke |
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| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Neurofilament Neurite Blotting Western Immunology Pathogenesis Immune system Dorsal root ganglion Ganglia Spinal Neurites medicine Animals Humans Immunology and Allergy Rats Wistar Fluorescent Antibody Technique Indirect Incubation Cells Cultured Aged Aged 80 and over biology business.industry Immune Sera Neural Inhibition Middle Aged Rats Blot medicine.anatomical_structure Neurology Antibody Formation Sensation Disorders biology.protein Female Neurology (clinical) Nervous System Diseases Antibody business |
| Zdroj: | Journal of Neuroimmunology. 74:165-172 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(96)00225-1 |
| Popis: | To investigate immune mechanisms in the etiology of idiopathic sensory neuronopathy (ISN), we studied neurite outgrowth inhibition and antibody binding to neuronal tissue of serum from 4 patients with ISN. Rat dorsal root ganglion (DRG) cells were cultured in the presence of serum from ISN patients and controls. After 48 h of incubation, neurite outgrowth was quantified with a neurofilament ELISA. Serum from ISN patients significantly inhibited DRG neurite outgrowth compared to controls. ISN serum also strongly immunostained fixed cultured and cryostat rat DRG neurons (at dilutions up to 1:10,240), whereas serum from controls did not. Western blots showed unique binding patterns to DRG proteins in 3 ISN patients compared with controls, but a single band corresponding in all ISN patients was not found. The inhibitory effect of ISN serum on neurite outgrowth and the presence of circulating anti-DRG antibodies in the acute phase of the disease supports an immune-mediated pathogenesis of ISN. |
| Databáze: | OpenAIRE |
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