Biocompatibility and modification of the protein-based adhesive secreted by the Australian frog Notaden bennetti
| Autor: | Noel K. Hart, Gail A. McFarland, Michael J. Tyler, Stephen J. Danon, John A. M. Ramshaw, Jerome A. Werkmeister, Margaret D. M. Evans, Russell J. Varley, Carl Braybrook, Graham Johnson, Lloyd D. Graham |
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| Rok vydání: | 2009 |
| Předmět: |
Exudate
Materials science Biocompatibility Surface Properties Biomedical Engineering Cell Culture Techniques Biocompatible Materials macromolecular substances Organ culture Gas Chromatography-Mass Spectrometry Biomaterials Acetone Cornea Tissue Culture Techniques Mice Cell Movement Adhesives Materials Testing medicine Cell Adhesion Organic chemistry Animals Carotenoid Cells Cultured chemistry.chemical_classification Mice Inbred BALB C biology Notaden technology industry and agriculture Metals and Alloys biology.organism_classification Resorption Endotoxins Biochemistry chemistry Cell culture Ceramics and Composites biology.protein Solvents Cattle Female medicine.symptom Anura Peroxidase |
| Zdroj: | Journal of biomedical materials research. Part A. 93(2) |
| ISSN: | 1552-4965 |
| Popis: | When provoked, Notaden bennetti frogs secrete a proteinaceous exudate, which rapidly forms a tacky and elastic glue. This material has potential in biomedical applications. Cultured cells attached and proliferated well on glue-coated tissue culture polystyrene, but migrated somewhat slower than on uncoated surfaces. In organ culture, dissolved glue successfully adhered collagen-coated perfluoropolyether lenses to debrided bovine corneas and supported epithelial regrowth. Small pellets of glue implanted subcutaneously into mice were resorbed by surrounding tissues, and all of the animals made a full recovery. An initial but transient skin necrosis at the implant site was probably caused by some of the potentially toxic metabolites present in the frog secretion; these include sterols and carotenoids, as well as fatty alcohols, aldehydes, ketones, acids, and aromatic compounds. Removal of the carotenoid pigments did not significantly alter the glue's material properties. In contrast, peroxidase treatment of dissolved glue introduced unnatural crosslinks between molecules of the major protein (Nb-1R) and resulted in the formation of a soft hydrogel, which was very different to the original material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010 |
| Databáze: | OpenAIRE |
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