The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation☆
| Autor: | Mohammad Hossein Nasr-Esfahani, Maryam Ostadsharif, Somayeh Tanhaie, Hossein Baharvand, Kamran Ghaedi, Khadijeh Karbalaie, Marzieh Mojbafan |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
Embryonal Carcinoma Stem Cells Transcription Genetic Neurogenesis education Retinoic acid Tretinoin Embryoid body Biology Mice chemistry.chemical_compound Cell Line Tumor Peroxisomes Animals Molecular Biology Peroxisomal matrix Gene Expression Profiling Cell Biology Peroxisome Catalase Molecular biology Embryonic stem cell Mice Inbred C57BL P19 cell chemistry Cell culture cardiovascular system circulatory and respiratory physiology Developmental Biology |
| Zdroj: | Differentiation. 81:127-132 |
| ISSN: | 0301-4681 |
| DOI: | 10.1016/j.diff.2010.11.003 |
| Popis: | Peroxisomal matrix protein is mainly expressed in heart, skeletal muscle, and brain tissues. To study the expression of peroxisomal protein (PEP) during neurogenesis, we employed mouse embryonic carcinoma cells (P19) and embryonic stem cells (mESCs) as an in vitro model for neural differentiation by retinoic acid (RA) induction. Expression pattern of PEP was investigated under distinct steps of differentiation by RT-PCR and real-time PCR. The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages. Therefore, we concluded that PEP might be involved in the early process of neurogenesis, which needs further verification. |
| Databáze: | OpenAIRE |
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