Steroid hormonal independence of HER-2/neu mRNA expression in four human ovarian carcinoma cell lines
| Autor: | Jolynda Jones, Leo D. Lagasse, Beth Y. Karlan |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
endocrine system diseases medicine.drug_class Receptor ErbB-2 Ovary Biology Adenocarcinoma Dexamethasone Ovarian carcinoma Internal medicine medicine Tumor Cells Cultured Humans Northern blot RNA Messenger Progesterone Ovarian Neoplasms Obstetrics and Gynecology Estrogens Genes erbB-2 medicine.disease Immunohistochemistry Blot Gene Expression Regulation Neoplastic Endocrinology medicine.anatomical_structure Oncology Receptors Estrogen Cell culture Estrogen Cancer research Female Ovarian cancer Receptors Progesterone |
| Zdroj: | Gynecologic oncology. 55(3 Pt 1) |
| ISSN: | 0090-8258 |
| Popis: | Overexpression of HER-2/ neu , a 185-kDa tyrosine kinase growth factor receptor, in human ovarian cancers has been correlated with a poor prognosis for survival of the disease. Previous studies have demonstrated that dexamethasone (Dex) induces a dose-dependent increase in HER-2/ neu mRNA levels in the ovarian cancer cell line SK-OV-3 by stabilizing the HER-2/ neu message. We extended these studies to test whether estrogen (Es), progesterone (Pr), and Dex were capable of regulating HER-2/ neu mRNA levels in the human ovarian cancer cell lines NIH:OVCAR-3, SW 626, OVCA 433, and Caov-3. Southern blotting demonstrated that all four cell lines contained a single copy of the 12.5-kb HER-2/ neu gene. Blotting techniques demonstrated low to barely detectable levels of HER-2/ neu mRNA and protein in these cell lines. To determine whether steroids regulated HER-2/ neu expression, all four ovarian cancer cell lines were cultured in the presence of 1 × 10 -7 M Es, Pr, or Dex and Northern blotting was completed. Unlike SK-OV-3 cells, the cell lines tested did not respond to the steroid treatments with alterations in their HER-2/ neu mRNA levels. In conclusion, neither Es, Pr, nor Dex regulates HER-2/ neu mRNA levels in NIH:OVCAR-3, SW 626, OVCA 433, and Caov-3 ovarian cancer cells. Future therapeutic manipulations of HER-2/ neu should not involve hormonal intervention. |
| Databáze: | OpenAIRE |
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