Serum detection of IgG antibodies against Demodex canis by western blot in healthy dogs and dogs with juvenile generalized demodicosis
| Autor: | Laia Solano Gallego, Mar Bardagí, Diana Ferreira, Ivan Ravera, Lluís Ferrer |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Mite Infestations
Mites General Veterinary biology medicine.diagnostic_test Blotting Western Pyoderma biology.organism_classification medicine.disease Immunoglobulin G Dogs Western blot Antigen parasitic diseases Immunology biology.protein medicine Demodicosis Animals Juvenile Dog Diseases Antibody Demodex |
| Zdroj: | Research in Veterinary Science. 101:161-164 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2015.06.011 |
| Popis: | The aim of this study was to investigate the presence of canine immunoglobulins (Ig) G against Demodex proteins in the sera of healthy dogs and of dogs with juvenile generalized demodicosis (CanJGD) with or without secondary pyoderma. Demodex mites were collected from dogs with CanJGD. Protein concentration was measured and a western blot technique was performed. Pooled sera from healthy dogs reacted mainly with antigen bands ranging from 55 to 72 kDa. Pooled sera from dogs with CanJGD without secondary pyoderma reacted either with 10 kDa antigen band or 55 to 72 kDa bands. Pooled sera from dogs with CanJGD with secondary pyoderma reacted only with a 10 kDa antigen band. The results of this study suggest that both healthy dogs and dogs with CanJGD develop a humoral response against different proteins of Demodex canis. |
| Databáze: | OpenAIRE |
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