Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae
| Autor: | Na Guo, Lu Yu, Fa Ge, Xuming Deng, Rizeng Meng, Xuelin Wang, Xiuping Wu, Jingbo Liu, Junwen Fan, Yi Yang |
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| Rok vydání: | 2009 |
| Předmět: |
Antifungal Agents
Microarray Genes Fungal Statistics as Topic Saccharomyces cerevisiae Bioengineering Applied Microbiology and Biotechnology Transcriptome Gene Expression Regulation Fungal Secondary metabolism Oligonucleotide Array Sequence Analysis biology Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Gene Expression Profiling Computational Biology biology.organism_classification Molecular biology Yeast Gene expression profiling Biochemistry Benzaldehydes DNA microarray Metabolic Networks and Pathways Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 37:313-322 |
| ISSN: | 1476-5535 1367-5435 |
| Popis: | p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge. |
| Databáze: | OpenAIRE |
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