Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia

Autor: Ola Didrik Saugstad, Máximo Vento, Leonid Pankratov, Ángel Sánchez-Illana, Julia Kuligowski, Anna Parra-Llorca, Rønnaug Solberg, Guillermo Quintás
Rok vydání: 2017
Předmět:
Male
Resuscitation
Liquid Chromatography - Time-of-Flight Mass Spectrometry (LC-TOF-MS)
Swine
Metabolite
Clinical Biochemistry
Metabolic biomarker
Severity of Illness Index
Biochemistry
Choline
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
030225 pediatrics
Severity of illness
Animals
Humans
Medicine
Hypoxia
lcsh:QH301-705.5
Asphyxia
lcsh:R5-920
Asphyxia Neonatorum
Hypoxanthine
business.industry
Organic Chemistry
Neonatal piglet model
Hypothermia
Hypoxia (medical)
Newborn
medicine.disease
Perinatal asphyxia
Liquid Chromatography – Time-of-Flight Mass Spectrometry (LC-TOF-MS)
Disease Models
Animal
lcsh:Biology (General)
Animals
Newborn
chemistry
Anesthesia
Hypoxia-Ischemia
Brain
Biomarker (medicine)
Female
medicine.symptom
lcsh:Medicine (General)
business
Biomarkers
030217 neurology & neurosurgery
Research Paper
Zdroj: Redox Biology
Redox Biology, Vol 12, Iss, Pp 1-7 (2017)
Publons
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
ISSN: 2213-2317
DOI: 10.1016/j.redox.2017.02.002
Popis: Hypoxic-ischemic encephalopathy (HIE) secondary to perinatal asphyxia is a leading cause of mortality and acquired long-term neurologic co-morbidities in the neonate. The most successful intervention for the treatment of moderate to severe HIE is moderate whole body hypothermia initiated within 6 h from birth. The objective and prompt identification of infants who are at risk of developing moderate to severe HIE in the critical first hours still remains a challenge. This work proposes a metabolite score calculated based on the relative intensities of three metabolites (choline, 6,8-dihydroxypurine and hypoxanthine) that showed maximum correlation with hypoxia time in a consolidated piglet model for neonatal hypoxia-ischemia. The metabolite score's performance as a biomarker for perinatal hypoxia and its usefulness for clinical grading and decision making have been assessed and compared to the performance of lactate which is currently considered the gold standard. For plasma samples withdrawn before and directly after a hypoxic insult, the metabolite score performed similar to lactate. However, it provided an enhanced predictive capacity at 2 h after resuscitation. The present study evidences the usefulness of the metabolite score for improving the early assessment of the severity of the hypoxic insult based on serial determinations in a minimally invasive biofluid. The applicability of the metabolite score for clinical diagnosis and patient stratification for hypothermia treatment has to be confirmed in multicenter trials involving newborns suffering from HIE.
Graphical abstract fx1
Databáze: OpenAIRE
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