Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx
| Autor: | Mary R. Hutson, Jarrett Burch, Asako K. Sato, Tony L. Creazzo, Harriett A. Stadt, Yin-Xiong Li, Margaret L. Kirby, Ping Zhang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cardiac neural crest
Heart Defects Congenital medicine.medical_specialty animal structures Persistent truncus arteriosus Fibroblast Growth Factor 8 Arterial pole Biology Fibroblast growth factor Heart development Antibodies Mice FGF8 Double outlet right ventricle Internal medicine FGF8 outflow tract Calcium transient medicine Morphogenesis Animals Molecular Biology Cardiac neural crest cells Neural crest Heart Cell Biology Anatomy medicine.disease Embryo Mammalian Truncus Arteriosus Persistent mkp3 Aorticopulmonary septum medicine.anatomical_structure Neural Crest embryonic structures Cardiology cardiovascular system Pharynx Secondary heart field Developmental Biology Signal Transduction |
| Zdroj: | Developmental biology. 295(2) |
| ISSN: | 0012-1606 |
| Popis: | Morphogenesis of the cardiac arterial pole is dependent on addition of myocardium and smooth muscle from the secondary heart field and septation by cardiac neural crest cells. Cardiac neural crest ablation results in persistent truncus arteriosus and failure of addition of myocardium from the secondary heart field leading to malalignment of the arterial pole with the ventricles. Previously, we have shown that elevated FGF signaling after neural crest ablation causes depressed Ca2+ transients in the primary heart tube. We hypothesized that neural crest ablation results in elevated FGF8 signaling in the caudal pharynx that disrupts secondary heart field development. In this study, we show that FGF8 signaling is elevated in the caudal pharynx after cardiac neural crest ablation. In addition, treatment of cardiac neural crest-ablated embryos with FGF8b blocking antibody or an FGF receptor blocker rescues secondary heart field myocardial development in a time- and dose-dependent manner. Interestingly, reduction of FGF8 signaling in normal embryos disrupts myocardial secondary heart field development, resulting in arterial pole malalignment. These results indicate that the secondary heart field myocardium is particularly sensitive to FGF8 signaling for normal conotruncal development, and further, that cardiac neural crest cells modulate FGF8 signaling in the caudal pharynx. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect