Mechanism of lipid bilayer perturbation by bactericidal membrane-active small molecules
| Autor: | A. Bortolotti, C. Troiano, S. Bobone, M.M. Konai, C. Ghosh, G. Bocchinfuso, Y. Acharya, V. Santucci, S. Bonacorsi, C. Di Stefano, J. Haldar, L. Stella |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Membranes
Small organic antimicrobial molecules Molecular dynamics simulations Lipid Bilayers Biophysics Cell Biology Biochemistry Anti-Bacterial Agents Mechanism of pore formation Anti-Infective Agents Settore CHIM/02 Liposomes Peptidomimetics Fluorescence spectroscopy Antimicrobial Cationic Peptides |
| Popis: | Membrane-active small molecules (MASMs) are small organic molecules designed to reproduce the fundamental physicochemical properties of natural antimicrobial peptides: their cationic charge and amphiphilic character. This class of compounds has a promising broad range of antimicrobial activity and, at the same time, solves some major limitations of the peptides, such as their high production costs and low in vivo stability. Most cationic antimicrobial peptides act by accumulating on the surface of bacterial membranes and causing the formation of defects when a threshold is reached. Due to the drastically different structures of the two classes of molecules, it is not obvious that small-molecule antimicrobials act in the same way as natural peptides, and very few data are available on this aspect. Here we combined spectroscopic studies and molecular dynamics simulations to characterize the mechanism of action of two different MASMs. Our results show that, notwithstanding their simple structure, these molecules act just like antimicrobial peptides. They bind to the membrane surface, below the head-groups, and insert their apolar moieties in the core of the bilayer. Like many natural peptides, they cause the formation of defects when they reach a high coverage of the membrane surface. In addition, they cause membrane aggregation, and this property could contribute to their antimicrobial activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|