Nanosized copper particles induced mesangial cell toxicity via the autophagy pathway
| Autor: | Pengjuan Xu, Min Cao, Xueqian Dong, Zhichao Yu, Jianwei Liu, Junzhen Tan, Yiyi Wang, Tao Li, Shuwu Zhao |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Physiology
Caspase 3 General Neuroscience Immunology Biophysics Ocean Engineering Apoptosis Cell Biology General Medicine Biochemistry Rats Nanosized copper particles (nano Cu) Cell Line Tumor Mesangial Cells Autophagy Animals Autophagy pathway Beclin-1 General Pharmacology Toxicology and Pharmaceutics Mesangial cells (MCs) Reactive Oxygen Species Autophagic flux Nephrotoxicity Microtubule-Associated Proteins Copper |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 55, Article number: e12252, Published: 11 NOV 2022 Brazilian Journal of Medical and Biological Research v.55 2022 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | Nanosized copper particles (nano Cu) have been incorporated into products in multiple industries, although studies have demonstrated that these particles are nephrotoxic. We investigated the cytotoxicity of nanosized copper particles on rat mesangial cells and measured rates of apoptosis, the expression of caspase-3, and generation of reactive oxygen species. We also measured autophagy through the acridine orange (AO) staining and expression of Beclin-1, microtubule-associated protein 1 light chain 3, and p62 to screen the underlying mechanism of toxicity. Nanosized copper particles inhibited mesangial cell viability, up-regulated the activity of caspase-3, and increased the rates of apoptosis and the generation of reactive oxygen species in a concentration-dependent manner. Exposure to nano Cu increased the formation of acidic vesicular organelles and the expression of Beclin-1, microtubule-associated protein 1 light chain 3, and p62, and treatment with an autophagy inhibitor reduced nephrotoxicity. This indicated that the autophagy pathway is involved in the toxicity induced by nanosized copper particles to mesangial cells. This finding can contribute to the development of safety guidelines for the evaluation of nanomaterials in the future. |
| Databáze: | OpenAIRE |
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