Modulatory activity of 9-hydroxy- and 9-hydroperoxy-octadecadienoic acid towards reactive oxygen species from guinea-pig pulmonary macrophages
| Autor: | Paul A.J. Henricks, Frans P. Nijkamp, Helen Van Der Vliet, Ferdi Engels |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Xanthine Oxidase Metabolite Linoleic acid Guinea Pigs In Vitro Techniques Biochemistry chemistry.chemical_compound Phagocytosis Superoxides Escherichia coli Animals Linoleic Acids Conjugated Phagocytic Cell Xanthine oxidase Calcimycin Cell Aggregation chemistry.chemical_classification Reactive oxygen species Chemistry Macrophages Zymosan Fatty acid Metabolism Hydrogen Peroxide Xanthine Kinetics Linoleic Acids Luminescent Measurements Fatty Acids Unsaturated Tetradecanoylphorbol Acetate |
| Zdroj: | European journal of biochemistry. 184(1) |
| ISSN: | 0014-2956 |
| Popis: | As guinea-pig pulmonary macrophages (PM) synthesize the linoleic acid metabolite 9-hydroxy-octadecadienoic acid (9-OH-Lin) under non-stimulated conditions in relatively large quantities, we investigated whether this product has an effect on the macrophage's own phagocytic cell function. 9-OH-Lin, and also its hydroperoxy precursor 9-hydroperoxy-octadecadienoic acid (9-OOH-Lin), influenced the generation of PM chemiluminescence, a measure of the production of reactive oxygen species. The generation of lucigenin-enhanced chemiluminescence by stimulated and non-stimulated PM was inhibited concentration-dependently. Inhibition was observed at concentrations as low as 10 nM. Since 9-OH-Lin and 9-OOH-Lin also inhibited the generation of chemiluminescence by a cell-free enzyme system, i.e. xanthine/xanthine oxidase, the inhibitory effects might represent a scavenging activity towards reactive oxygen species. 9-OH-Lin and 9-OOH-Lin did not influence other phagocytic cell functions, e.g. PM phagocytic capacity, the aggregatory response to the calcium ionophore A23187, or the release of lysosomal enzymes. The effects of 9-OH-Lin and 9-OOH-Lin could be ascribed to the hydroxy and hydroxyperoxy moiety, respectively, as evidenced by lack of effect of the native fatty acid linoleic acid. We conclude that the formation of 9-OH-Lin and 9-OOH-Lin by PM may represent a regulatory mechanism towards the cell's own activity by modulating reactive oxygen species. |
| Databáze: | OpenAIRE |
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