Different epitopes of Ralstonia solanacearum effector RipAW are recognized by two Nicotiana species and trigger immune responses
| Autor: | Yue Chen, Xin Jin, Cuizhu Zhao, Mingxia Lu, Yong Zhang, Núria S. Coll, Haibin Lu, Yang Niu, Yizhe He, Jinxue Hu, Qin Chen, Dongdong Wang, Mancang Zhang, Marc Valls, Huijuan Wang, Yisa Wang, Shouyang Fu, Gong Chen |
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| Přispěvatelé: | Natural Science Foundation of Shaanxi Province, Shaanxi Provincial Key Laboratory Project of Department of Education, Shaanxi Province, National Natural Science Foundation of China |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Cell death
Nicotiana viruses Nicotiana tabacum Bacterial diseases Soil Science Nicotiana benthamiana Plant cells and tissues Plant Science Effector-triggered immunity medicine.disease_cause Epitope Epitopes Protein Domains effector‐triggered immunity Tobacco medicine Plant Immunity Molecular Biology E3 ligase Plant Diseases Ralstonia solanacearum Mutation Malalties bacterianes biology Effector RipAW fungi food and beverages Original Articles biology.organism_classification Cèl·lules i teixits vegetals Cell biology cell death effector Immune system Sistema immunitari Original Article Agronomy and Crop Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de la UB Universidad de Barcelona Molecular Plant Pathology |
| ISSN: | 2020-2210 |
| Popis: | Diverse pathogen effectors convergently target conserved components in plant immunity guarded by intracellular nucleotide-binding domain leucine-rich repeat receptors (NLRs) and activate effector-triggered immunity (ETI), often causing cell death. Little is known of the differences underlying ETI in different plants triggered by the same effector. In this study, we demonstrated that effector RipAW triggers ETI on Nicotiana benthamiana and Nicotiana tabacum. Both the first 107 amino acids (N1-107) and RipAW E3-ligase activity are required but not sufficient for triggering ETI on N. benthamiana. However, on N. tabacum, the N1-107 fragment is essential and sufficient for inducing cell death. The first 60 amino acids of the protein are not essential for RipAW-triggered cell death on either N. benthamiana or N. tabacum. Furthermore, simultaneous mutation of both R75 and R78 disrupts RipAW-triggered ETI on N. tabacum, but not on N. benthamiana. In addition, N. tabacum recognizes more RipAW orthologs than N. benthamiana. These data showcase the commonalities and specificities of RipAW-activated ETI in two evolutionally related species, suggesting Nicotiana species have acquired different abilities to perceive RipAW and activate plant defences during plant–pathogen co-evolution. This work was funded by the Nature Science Basic Research Plan of Shaanxi Province (2020JM-160), the Special Research project from Shaanxi province (F2020221001), the Program of Introducing Talents of Innovative Discipline to Universities (Project 111) from the State administration of Foreign Experts Affairs (B18042) and the External Science and Technology Cooperation Program of Ningxia Academy of Agriculture and Forestry Sciences (DW-X-2018012), the Start-up Funds of Northwest A&F University (Z111021601), the Fundamental Research Fund for the Central Universities of China (Z109021706), the Key Research and Development plan of Ningxia Province (2021BEF02033), and the National Natural Science Foundation of China for C.Z. (31901573). |
| Databáze: | OpenAIRE |
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