Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut
| Autor: | Maria Louise Leth, Signe Schultz Pedersen, Maher Abou Hachem, Morten Ejby, Finn Lillelund Aachmann, David Adrian Ewald, Christopher T. Workman, Martin Iain Bahl, Bjørge Westereng, Claus Sternberg, Tine Rask Licht |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Dietary Fiber
0301 basic medicine Microbiology (medical) Glycan animal structures media_common.quotation_subject Immunology Applied Microbiology and Biotechnology Microbiology Substrate Specificity 03 medical and health sciences Bacterial Proteins SDG 3 - Good Health and Well-being Genetics Bacteroides Humans Symbiosis Internalization media_common Clostridiales Endo-1 4-beta Xylanases biology food and beverages Transporter Gene Expression Regulation Bacterial Cell Biology biology.organism_classification Commensalism Xylan Coculture Techniques Gastrointestinal Microbiome Transport protein carbohydrates (lipids) 030104 developmental biology Biochemistry Xylanase biology.protein ATP-Binding Cassette Transporters Xylans |
| Zdroj: | Nature Microbiology Leth, M L, Ejby, M, Workman, C, Ewald, D A, Pedersen, S S, Sternberg, C, Bahl, M I, Licht, T R, Aachmann, F L, Westereng, B & Abou Hachem, M 2018, ' Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut ', Nature Microbiology . https://doi.org/10.1038/s41564-018-0132-8 |
| DOI: | 10.1038/s41564-018-0132-8 |
| Popis: | Metabolism of dietary glycans is pivotal in shaping the human gut microbiota. However, the mechanisms that promote competition for glycans among gut commensals remain unclear. Roseburia intestinalis, an abundant butyrate-producing Firmicute, is a key degrader of the major dietary fibre xylan. Despite the association of this taxon to a healthy microbiota, insight is lacking into its glycan utilization machinery. Here, we investigate the apparatus that confers R. intestinalis growth on different xylans. R. intestinalis displays a large cell-attached modular xylanase that promotes multivalent and dynamic association to xylan via four xylan-binding modules. This xylanase operates in concert with an ATP-binding cassette transporter to mediate breakdown and selective internalization of xylan fragments. The transport protein of R. intestinalis prefers oligomers of 4–5 xylosyl units, whereas the counterpart from a model xylan-degrading Bacteroides commensal targets larger ligands. Although R. intestinalis and the Bacteroides competitor co-grew in a mixed culture on xylan, R. intestinalis dominated on the preferred transport substrate xylotetraose. These findings highlight the differentiation of capture and transport preferences as a possible strategy to facilitate co-growth on abundant dietary fibres and may offer a unique route to manipulate the microbiota based on glycan transport preferences in therapeutic interventions to boost distinct taxa. Characterization of xylan utilization loci in the butyrate-producing Firmicute Roseburia intestinalis provides mechanistic insight into its growth on different xylan substrates and its ability to co-grow and compete with a xylan-degrading commensal from the Bacteroides genus. |
| Databáze: | OpenAIRE |
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