Polymer-coated echogenic lipid nanoparticles with dual release triggers
| Autor: | Sanku Mallik, Kalpana S. Katti, Rahul Nahire, Manas K. Haldar, Kausik Sarkar, Kara N. Gange, Avinash H. Ambre, D. K. Srivastava, Anaas Mergoum, Shirshendu Paul |
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| Rok vydání: | 2013 |
| Předmět: |
Polymers and Plastics
Dual release Cell Survival Polymers Nanoparticle Bioengineering Nanotechnology Microscopy Atomic Force Article Biomaterials Drug Delivery Systems Folic Acid Coated Materials Biocompatible Microscopy Electron Transmission Materials Chemistry medicine Humans Doxorubicin Particle Size chemistry.chemical_classification Drug Carriers Chemistry Polymer Lipids Target site Drug delivery MCF-7 Cells Nanoparticles Particle size Drug carrier medicine.drug HeLa Cells |
| Zdroj: | Biomacromolecules. 14(3) |
| ISSN: | 1526-4602 |
| Popis: | Although lipid nanoparticles are promising drug delivery vehicles, passive release of encapsulated contents at the target site is often slow. Herein, we report contents release from targeted, polymer coated, echogenic lipid nanoparticles in the cell cytoplasm by redox trigger and simultaneously enhanced by diagnostic frequency ultrasound. The lipid nanoparticles were polymerized on the external leaflet using a disulfide cross-linker. In the presence of cytosolic concentrations of glutathione, the lipid nanoparticles released 76% of encapsulated contents. Plasma concentrations of glutathione failed to release the encapsulated contents. Application of 3 MHz ultrasound for 2 minutes simultaneously with the reducing agent enhanced the release to 96%. Folic acid conjugated, doxorubicin loaded nanoparticles showed enhanced uptake and higher cytotoxicity in cancer cells overexpressing the folate receptor (compared to the control). With further developments, these lipid nanoparticles have the potential to be used as multimodal nanocarriers for simultaneous targeted drug delivery and ultrasound imaging. |
| Databáze: | OpenAIRE |
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