A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
| Autor: | Cliff E. Foster, Kristoffer A. Meunier, Jackson Gehan, Renee M. Happs, Anne E. Harman-Ware, Fachuang Lu, Fengxia Yue, Crissa Doeppke, Mark F. Davis |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Glycerol Poaceae 01 natural sciences Applied Microbiology and Biotechnology Lignin Cell wall structure Gas Chromatography-Mass Spectrometry Biotech Methods chemistry.chemical_compound 010608 biotechnology Calibration Sulfhydryl Compounds Throughput (business) Chromatography Thioacidolysis Chemistry Organic solvent S/G ratio General Medicine Wood Biotech Method Reaction product High-Throughput Screening Assays Monomer Molecular Medicine Biomass fuels Quantitative analysis (chemistry) 010606 plant biology & botany |
| Zdroj: | Biotechnology Journal |
| ISSN: | 1860-7314 1860-6768 |
| Popis: | Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β‐O‐4 linkages. Current thioacidolysis methods are low‐throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non‐chlorinated organic solvent and is tailored for higher‐throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1–2 mg of biomass per assay and has been quantified using fast‐GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day‐to‐day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses. |
| Databáze: | OpenAIRE |
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