The Inhibitory Effect of Preeclamptic Umbilical Cord Blood Serum on Matrix Metalloproteinase-1 in Arterial Slices Incubated in vitro
| Autor: | Tomasz Gogiel, Lech Romanowicz, Edward Bańkowski, Stefan Jaworski, Zofia Galewska |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Blotting Western Enzyme-Linked Immunosorbent Assay Gestational Age Matrix metalloproteinase Umbilical cord Umbilical Arteries Pathology and Forensic Medicine Preeclampsia Tissue Culture Techniques Blood serum Pre-Eclampsia Pregnancy Internal medicine medicine Humans Molecular Biology reproductive and urinary physiology Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinase-1 Chemistry Cell Biology General Medicine Fetal Blood medicine.disease female genital diseases and pregnancy complications In vitro Blot Endocrinology medicine.anatomical_structure embryonic structures Immunology Collagenase Female Matrix Metalloproteinase 1 Umbilical Cord Serum medicine.drug |
| Zdroj: | Pathobiology. 73:310-316 |
| ISSN: | 1423-0291 1015-2008 |
| Popis: | Background: Our previous studies demonstrated that preeclampsia is accompanied by significant alterations in the amounts of peptide growth factors in the umbilical cord serum. Some of these factors (especially IGF-1) are known as regulators of collagen metabolism. The umbilical cord arteries (UCAs) of newborns delivered by mothers with preeclampsia contain more than twice the amount of collagen in comparison to newborns delivered by healthy mothers. A significant role in collagen degradation is attributed to matrix metalloproteinase (MMP)-1 (collagenase 1) and tissue inhibitors of metalloproteinases (TIMPs). Objective: To compare the effects of umbilical cord (UC) blood serum of control and preeclamptic newborns on the content and activity of MMP-1, TIMP-1 and TIMP-2 in UCA wall slices incubated in vitro. Methods: Polyacrylamide gel electrophoresis (PAGE) followed by Western immunoblotting allowed to detect MMP-1 as well as TIMP-1 and TIMP-2. The amounts of MMP-1, TIMP-1 and TIMP-2 in UCA slices were measured by immunoenzymatic method (ELISA). MMP-1 activity in the arterial wall was measured using a collagenase-1-specific substrate. Results: Western immunoblot analyses detected MMP-1, TIMP-1 and TIMP-2 in the incubation fluids and in extracts from the UCA wall. Both 43- and 55-kDa (a zymogen) bands of MMP-1 were visible. The control UC serum stimulated both the amount as well as actual and potential activities of MMP-1 in the arterial wall in a time-dependent manner. In contrast to controls, the preeclamptic serum did not exert such an effect. Conclusions: The small amount and low activity of MMP-1 accompanied by elevated amounts of TIMPs (especially TIMP-1) decelerate the degradation and enhance the accumulation of collagen in the preeclamptic UCA wall. |
| Databáze: | OpenAIRE |
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