Characterization of the P2 receptors in rabbit pulmonary artery
| Autor: | Peter S. Macdonald, Owe-Young R, C. G. Schyvens, R A Qasabian, J P Killen, Arthur D. Conigrave, D J Williamson |
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| Rok vydání: | 1997 |
| Předmět: |
Male
P2Y receptor medicine.medical_specialty Vascular smooth muscle Endothelium Suramin Vasodilation Uridine Triphosphate Biology In Vitro Techniques Pulmonary Artery Nitric Oxide Nitroarginine Muscle Smooth Vascular chemistry.chemical_compound Adenosine Triphosphate Internal medicine medicine Animals Receptor Cells Cultured Pharmacology Dose-Response Relationship Drug Receptors Purinergic P1 Endothelial stem cell medicine.anatomical_structure Endocrinology chemistry Papers cardiovascular system Calcium Female Endothelium Vascular Rabbits medicine.drug |
| Zdroj: | British journal of pharmacology. 120(4) |
| ISSN: | 0007-1188 |
| Popis: | 1 We have identified the P2 receptors mediating vasomotor responses in the rabbit pulmonary artery. 2 Neither ATP nor UTP contracted intact or endothelium-denuded rings. However, both relaxed intact rings of rabbit pulmonary artery that had been preconstricted with phenylephrine (pD2 5.2 and 5.6, respectively). 3 The vasodilator effect of UTP was endothelium-dependent and abolished by the nitric oxide synthase inhibitor NG-nitro-L-arginine (L-NOARG). 4 The vasodilator effect of ATP was only partially inhibited by removal of endothelium or addition of L-NOARG, suggesting an additional direct effect on vascular smooth muscle. 5 The endothelium-dependent vasodilator responses to UTP and ATP were competitively antagonized by suramin. 6 Preconstricted, endothelium-denuded rings were also relaxed by 2-methylthio ATP (pD2 6.6), a P2Y receptor agonist. 7 Ca2+-mobilizing P2U receptors were identified on smooth muscle cells on the basis of single cell responses to ATP (pD2 7.8) and UTP (pD2 7.9; 6.7 in the presence of 100 μm suramin). 8 There was no evidence of a Ca2+-mobilizing P2Y receptor in these cultured cells. 9 The data suggest the presence of (i) a suramin-sensitive P2U receptor on endothelial cells that induces vasorelaxation through NO release, (ii) a suramin-sensitive P2U receptor on cultured smooth muscle cells that mobilizes Ca2+ but is not coupled to vasomotor responses and (iii) a putative P2Y receptor on vascular smooth muscle cells that induces relaxation via a Ca2+-independent signal transduction pathway. |
| Databáze: | OpenAIRE |
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