Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients
| Autor: | Inmaculada Ventura, Rocío Aroca, M Prados, Javier Monteseirín, Bobadilla P, Antonio Vega-Rioja, Ricardo Palacios, Cristina Chamorro, Lourdes Fernández-Delgado, David Rodriguez |
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| Přispěvatelé: | [Fernández-Delgado,L, Vega-Rioja,A, Ventura,I, Chamorro,C, Aroca,R, Prados,M, Monteseirín,J] Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain. [Monteseirín,J] Departamento de Medicina. Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Bobadilla,P] Servicio de Alergia, Hospital Infanta Elena, Badajoz, Spain. [Rodríguez,D, Palacios,R] Laboratorios Diater, Madrid, Spain., This work was supported by the FIS-Thematic Networks and Co-Operative Research Centres RIRAAF (RD07-0064) and Fundación Alergol, Spain. Javier Monteseirín is under the Programa de Intensificación de la Actividad Investigadora del Sistema Nacional de Salud. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Allergy Galectina 3 Neutrophils Galectin 3 lcsh:Medicine medicine.disease_cause Immunoglobulin E Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Allergen Alérgenos Phenomena and Processes::Biological Phenomena::Biological Processes [Medical Subject Headings] Receptor lcsh:Science Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings] Multidisciplinary biology Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Lectins::Galectins::Galectin 3 [Medical Subject Headings] Lactoferrin food and beverages Middle Aged Female medicine.symptom Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors::Immunosuppressive Agents [Medical Subject Headings] Research Article Adult Adolescent Inflammation Diseases::Respiratory Tract Diseases::Lung Diseases::Lung Diseases Obstructive::Asthma [Medical Subject Headings] Young Adult medicine Humans Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies Monoclonal [Medical Subject Headings] Asma Asthma Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Lactoferrin [Medical Subject Headings] business.industry Receptors IgE lcsh:R Allergens medicine.disease Inmunosupresores In vitro Case-Control Studies Anticuerpos monoclonales Immunology biology.protein Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Membrane Proteins::Receptors Cell Surface::Receptors Immunologic::Receptors Fc::Receptors IgE [Medical Subject Headings] lcsh:Q Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings] Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils [Medical Subject Headings] business Chemicals and Drugs::Biological Factors::Inflammation Mediators [Medical Subject Headings] |
| Zdroj: | PLoS ONE, Vol 10, Iss 10, p e0141278 (2015) PLoS ONE Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid |
| ISSN: | 1932-6203 |
| Popis: | Journal Article; BACKGROUND Despite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question. METHODS Neutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed. RESULTS Lf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients. Yes |
| Databáze: | OpenAIRE |
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