Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin
| Autor: | Cecı´lia Carvalho de Oliveira, Mário Rogério Lima Mota, Tatiane Santi-Gadelha, Ana Maria Sampaio Assreuy, Carlos Alberto de Almeida Gadelha, Raphaela Cardoso Gomes, Karoline S. Aragão, Benildo Sousa Cavada, Marcos H. Toyama, David N. Criddle, Nylane M.N. Alencar, Daniela de Oliveira Toyama, Alana de Freitas Pires |
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| Rok vydání: | 2006 |
| Předmět: |
Ion chromatography
Biophysics Biology Biochemistry Divalent chemistry.chemical_compound Affinity chromatography Chitin Glucosamine Animals Molecular Biology chemistry.chemical_classification Inflammation Chromatography Bacteria Dose-Response Relationship Drug Plant Extracts Lectin Cell Biology biology.organism_classification Rats Cycadopsida chemistry Seeds biology.protein Plant Lectins Antibacterial activity |
| Zdroj: | Biochemical and biophysical research communications. 350(4) |
| ISSN: | 0006-291X |
| Popis: | This paper describes the purification and characterization of a new N-acetyl-D-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0 kDa (SDS-PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-D-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes. |
| Databáze: | OpenAIRE |
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