Stability-indicating analytical approach for stability evaluation of lactoferrin
| Autor: | Timeja Planinšek Parfant, Nika Osel, Robert Roškar, Albin Kristl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
udc:66.022.362:535.33/.34:615
Pharmaceutical Science proteini stabilnost laktoferin nadzor kakovosti Intrinsic fluorescence udc:66.02 01 natural sciences Article Pharmacy and materia medica 0404 agricultural biotechnology stabilnost Stability indicating Bicinchoninic acid assay Spektroskopska analiza quality control laktoferin lactoferrin stability HPLC-UV spectroscopic methods stability-indicating approach proteins quality control nadzor kakovosti Chromatography HPLC-UV biology Lactoferrin Chemistry spectroscopic methods 010401 analytical chemistry A protein stability‑indicating approach 04 agricultural and veterinary sciences stability 040401 food science proteins 0104 chemical sciences lactoferrin spektroskopska analiza RS1-441 HPLC‑UV biology.protein stability-indicating approach proteini |
| Zdroj: | Pharmaceutics, vol. 13, no. 7, 1065, 2021. Pharmaceutics, str. 1-24 : Ilustr., Vol. 13, iss. 7, 2021 COBISS-ID: 517949977 Pharmaceutics Pharmaceutics, Vol 13, Iss 1065, p 1065 (2021) Volume 13 Issue 7 |
| ISSN: | 1999-4923 |
| Popis: | Lactoferrin is a multifunctional iron-binding glycoprotein in milk. Due to its potential for the treatment of various diseases, interest in products containing lactoferrin is increasing. However, as a protein, it is prone to degradation, which critically affects the quality of products. Therefore, the main purpose of our work was to develop a stability-indicating analytical approach for stability evaluation of lactoferrin. We were focused on two complementary methods: reversed-phase and size-exclusion chromatography. The stability-indicating nature of the selected methods was confirmed. They were successfully validated by following the ICH guidelines and applied to preliminary lactoferrin stability studies. Up to three degradation products, as well as aggregates and fragments of lactoferrin, were detected in various samples using complementary reversed-phase and size-exclusion chromatographic methods. The analytical approach was additionally extended with three spectroscopic techniques (absorbance, intrinsic fluorescence, and bicinchoninic acid method), which may provide valuable complementary information in some cases. The presented analytical approach allows the stability evaluation of lactoferrin in various samples, including the ability to detect differences in its degradation mechanisms. Furthermore, it has the potential to be used for the quality control of products containing lactoferrin. Nasl. z nasl. zaslona. Opis vira z dne 13. 7. 2021. Št. članka: 1065. Abstract. Bibliografija: str. 23-24. ARRS Support of Research and development projects (TRL 3–6), S4—Networks for the transition to a circular economy, Biomass and alternative raw materials, Fractionation and processing of whey proteins and exploitation of the residue for the formation of new functional foods and food supplements, LAKTIKA |
| Databáze: | OpenAIRE |
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