Natural Killer Cell Functional Activity Suppression By Intravenous Immunoglobulin, Intralipid and Soluble Human Leukocyte Antigen-G
| Autor: | Roumen G. Roussev, Siu Chui Ng, Carolyn B. Coulam |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Abortion
Habitual Fat Emulsions Intravenous Immunology Fluorescent Antibody Technique Human leukocyte antigen Biology Pharmacology Natural killer cell Flow cytometry HLA Antigens Pregnancy medicine Humans Immunology and Allergy Cytotoxicity Cells Cultured HLA-G Antigens medicine.diagnostic_test Histocompatibility Antigens Class I Immunoglobulins Intravenous Obstetrics and Gynecology Flow Cytometry In vitro Killer Cells Natural medicine.anatomical_structure Reproductive Medicine biology.protein Functional activity Female Antibody K562 cells |
| Zdroj: | American Journal of Reproductive Immunology. 57:262-269 |
| ISSN: | 1600-0897 1046-7408 |
| DOI: | 10.1111/j.1600-0897.2007.00473.x |
| Popis: | Problem The purpose of this study was to compare the ability of intravenous immunoglobulin (IVIg), intralipid and soluble human leukocyte antigen (sHLA)-G to suppress natural killer (NK) cell cytotoxicity in an in vitro assay. Method of study Blood samples taken from 275 women experiencing reproductive failure were analyzed for NK cytotoxicity and the suppression of NK cytotoxicity by IVIg 4 and 2 mg/mL (n = 275), intralipid 18 and 9 mg/mL (n = 275) and sHLA-G 70 and 35 ng/mL (n = 50) using immunofluorescent labeled K562 cells as targets and flow cytometry. Results Natural killer cytotoxicity was suppressed in all samples. Among patients with normal NK cell activity, IVIg suppressed NK cytotoxicity by 44.9 ± 8.1%, intralipid suppressed NK killing by 45.2 ± 8.3% and sHLA-G suppressed by 49.0 ± 9.2%. When specimens with abnormal NK activity were observed for suppression of cytotoxicity, IVIg suppressed by 38.9 ± 5.4%, intralipid suppressed by 39.8 ± 6.2% and sHLA-G suppressed by 39.9 ± 5.0%. Conclusion Intravenous immunoglobulin, intralipid and sHLA-G suppressed NK cell cytotoxicity with equal efficacy in an in vitro assay. |
| Databáze: | OpenAIRE |
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