SPANosomes as Delivery Vehicles for Small Interfering RNA (siRNA)
| Autor: | Yicheng Mao, Chenguang Zhou, Robert W. Brueggemeier, Yasuro Sugimoto, Bo Yu, Robert J. Lee, Naveen Kanthamneni, Yue Zhang, L. James Lee |
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| Rok vydání: | 2011 |
| Předmět: |
Small interfering RNA
Green Fluorescent Proteins Pharmaceutical Science Biology Transfection Article Polyethylene Glycols Green fluorescent protein law.invention Fatty Acids Monounsaturated Surface-Active Agents Aromatase Microscopy Electron Transmission Confocal microscopy law Cell Line Tumor Drug Discovery Gene Knockdown Techniques Humans Vitamin E Particle Size RNA Small Interfering Hexoses Gene knockdown Liposome Cryoelectron Microscopy Flow Cytometry Molecular biology Cell biology Quaternary Ammonium Compounds Liposomes Drug delivery Nanoparticles Molecular Medicine Oligonucleotide Probes |
| Zdroj: | Molecular Pharmaceutics. 9:201-210 |
| ISSN: | 1543-8392 1543-8384 |
| Popis: | Nonionic surfactant vesicles, or SPANosomes (SPs), comprised of cationic lipid and sorbitan monooleate (Span 80) were synthesized and evaluated as small interfering RNA (siRNA) vectors. The SPs had a mean diameter of less than 100 nm and exhibited excellent colloidal stability. The SP/siRNA complexes possessed a slightly positive zeta potential of 12 mV and demonstrated a high siRNA incorporation efficiency of greater than 80%. Cryogenic transmission electron microscopy (cryo-TEM) imaging of the SP/siRNA indicated a predominantly core-shell structure. The SP/siRNA complexes were shown to efficiently and specifically silence expression of both green fluorescent protein (GFP) (66% knockdown) and aromatase (77% knockdown) genes in breast cancer cell lines. In addition, the cellular trafficking pathway of the SP/siRNA was investigated by confocal microscopy using molecular beacons as probes for cytosolic delivery. The results showed efficient endosomal escape and cytosolic delivery of the siRNA cargo following internalization of the SP/siRNA complexes. In conclusion, Span 80 is a potent helper lipid, and the SPs are promising vehicles for siRNA delivery. |
| Databáze: | OpenAIRE |
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