Engineering of quorum-sensing systems for improved production of alkaline protease by Bacillus subtilis
| Autor: | R. Daskin, E.J. Koetje, S Bron, M.J.M. Kolkman, Oscar P. Kuipers, Eugenio Ferrari, Harold Tjalsma, R. Kiewiet, J.H. van der Laan |
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| Přispěvatelé: | Molecular Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
EXPRESSION
animal structures Transcription Genetic education Gene Expression DEGU Bacillus subtilis PHOSPHORELAY Applied Microbiology and Biotechnology quorum-sensing SIGNAL-TRANSDUCTION PATHWAY GENETIC COMPETENCE Bioreactors Bacterial Proteins protein secretion Extracellular AprE Overproduction SPORULATION Molecular diagnosis prognosis and monitoring [UMCN 1.2] Bacillaceae biology Serine Endopeptidases fungi Proteolytic enzymes Membrane Transport Proteins General Medicine biology.organism_classification Bacillales Quorum sensing Response regulator Rap-Phr Biochemistry DEGRADATIVE ENZYME-SYNTHESIS RESPONSE REGULATOR PHOSPHATASES Genetic Engineering gene regulation INTEGRATION Biotechnology |
| Zdroj: | Journal of Applied Microbiology, 96(3), 569-578. Wiley Journal of Applied Microbiology, 96, 3, pp. 569-78 Journal of Applied Microbiology, 96, 569-78 |
| ISSN: | 1364-5072 |
| DOI: | 10.1111/j.1365-2672.2004.02179.x |
| Popis: | H. T JALSMA, E .J. K OETJE, R . K IEWIET, O .P. K UIPERS, M . K OLKMAN, J . V AN D ER L AAN, R . D ASKIN, E . F E R R A R I A N D S . B R O N . 2004. Aim: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. Methods and Results: Addition of synthetic Phr pentapeptides to the growth medium, or overproduction of prePhr peptides, slightly improved the transcription of the aprE gene in B. subtilis. Disruption of certain rap genes similarly improved the transcription of the aprE gene. The production of extracellular proteolytic enzymes was increased when the rapA mutation was combined with a degU32 (Hy) mutation for hyper-secretion. Conclusions: Certain Rap-Phr systems of B. subtilis seem to suppress extracellular AprE production. Although this may be an important feature under natural conditions, repression of AprE production by these systems is not desirable under fermentation conditions. Significance and Impact of the Study: Although the levels of aprE transcriptional increase in this study are moderate, engineering of Rap-Phr systems may be used to improve the yield of Bacillus strains that are used for the production of the extracellular protease AprE, or Bacillus strains that use of the aprE promoter for the production of a heterologous protein. |
| Databáze: | OpenAIRE |
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