Glucose Transporters, Hexokinase, and Phosphofructokinase in Brain of Rats with Perinatal Asphyxia
| Autor: | Barbara Lubec, Erwin Kitzmueller, Harald Hoeger, Marina Chiappe-Gutierrez, Gert Lubec |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Monosaccharide Transport Proteins Phosphofructokinase-1 Blotting Western Biology Rats Sprague-Dawley chemistry.chemical_compound Pregnancy Hexokinase Internal medicine medicine Animals Humans RNA Messenger Asphyxia Asphyxia Neonatorum Infant Newborn Glucose transporter Brain Hydrogen-Ion Concentration medicine.disease Rats Perinatal asphyxia Glucose Endocrinology chemistry Cerebral blood flow Regional Blood Flow Pediatrics Perinatology and Child Health biology.protein Female GLUT1 medicine.symptom GLUT3 Phosphofructokinase |
| Zdroj: | Pediatric Research. 47:84-84 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-200001000-00016 |
| Popis: | Transport by glucose transporters from blood to the brain during hypoxic-ischemic conditions is well studied. However, the recent availability of a clinically related animal model of perinatal asphyxia and the fact that no concomitant determination of glucose transporters, parameters for glucose utilization, brain glucose, and cerebral blood flow (CBF) have been reported and the early phase of perinatal asphyxia has never been studied led us to perform the following study. Cesarean section was performed on full-term pregnant rats. The obtained pups within patent uterus horns were placed into a water bath at 37 degrees C from which they were subsequently removed after 5-20 min of graded asphyxia. Brain pH, brain tissue glucose, CBF, mRNA and activity of hexokinase and phosphofructokinase, and mRNA and protein of the glucose transporters GLUTI and GLUT3 were determined. Brain pH decreased and brain tissue glucose and CBF increased with the length of the asphyctic period; hexokinase and phosphofructokinase mRNA and activity were unchanged during the observation period. The mRNA and protein of both glucose transporters were comparable between normoxic and asphyctic groups. We show that glucose transport and utilization are unchanged in the early phase of perinatal asphyxia at a time point when CBF and brain glucose are already significantly increased and severe acidosis is present. |
| Databáze: | OpenAIRE |
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