Diglycolic acid induces HepG2/C3A liver cell toxicity in vitro
| Autor: | Vanessa Topping, Kathleen Belgrave, Howard Toomer, Jessica Sprando, Nicholas Olejnik, Zachary Keltner, Paddy L. Wiesenfeld, Ana Depina, Sanah Vohra, Robert L. Sprando, Thomas J. Flynn, Shelia Pugh-Bishop, Miriam E. Mossoba, T.N. Black |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Cell Survival Diglycolic acid Cell Cycle Proteins Pharmacology Toxicology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food In vivo Animals Humans Ingestion Membrane Potential Mitochondrial Dose-Response Relationship Drug Liver cell Food additive Hepatotoxin Nuclear Proteins Reproducibility of Results Hep G2 Cells General Medicine In vitro Glycolates 030104 developmental biology Liver chemistry 030220 oncology & carcinogenesis Toxicity Food Additives Multidrug Resistance-Associated Proteins Reactive Oxygen Species Heme Oxygenase-1 |
| Zdroj: | Toxicology in Vitro. 52:87-93 |
| ISSN: | 0887-2333 |
| Popis: | Carboxymethyl starches are added to food products for thickening or tablet binding/filling purposes. Although they lack toxicity, their synthesis creates the chemical byproduct diglycolic acid (DGA), which is difficult to eliminate and whose toxicity is in question. A rare case of an accidental direct exposure to extremely high concentrations of DGA in a person revealed that DGA has the potential to be toxic to several organs, with the kidneys and liver being the most affected organs. Given that DGA is present in our food supply as a chemical byproduct of carboxymethyl starch food additives, we sought to perform in vitro testing of its potential hepatotoxicity to help complement a recent in vivo rat acute dose-response study that also tested for the potential hepatotoxic effects of daily DGA ingestion by oral gavage over a period of 28 days. Using the HepG2/C3A cellular in vitro model, we tested how escalating doses of DGA exposure over 24 h could induce hepatotoxicity. Both in vitro and in vivo testing systems revealed that DGA is indeed a hepatotoxin once a certain exposure threshold is reached. The concordance of these models highlights the utility of in vitro testing to support and help predict in vivo findings. |
| Databáze: | OpenAIRE |
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